{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:30:26Z","timestamp":1790760626415,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T00:00:00Z","timestamp":1689552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["20\u201019162S"],"award-info":[{"award-number":["20\u201019162S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["00023736"],"award-info":[{"award-number":["00023736"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010099","name":"\u00dastav organick\u00e9 chemie a biochemie Akademie v\u011bd \u010cesk\u00e9 republiky","doi-asserted-by":"publisher","award":["RVO 61388963"],"award-info":[{"award-number":["RVO 61388963"]}],"id":[{"id":"10.13039\/501100010099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:p>Mutations in the splicing factor 3b subunit 1 (<jats:italic>SF3B1<\/jats:italic>) gene are frequent in myelodysplastic neoplasms (MDS). Because the splicing process is involved in the production of circular RNAs (circRNAs), we investigated the impact of <jats:italic>SF3B1<\/jats:italic> mutations on circRNA processing. Using RNA sequencing, we measured circRNA expression in CD34+ bone marrow MDS cells. We defined circRNAs deregulated in a heterogeneous group of MDS patients and described increased circRNA formation in higher\u2010risk MDS. We showed that the presence of <jats:italic>SF3B1<\/jats:italic> mutations did not affect the global production of circRNAs; however, deregulation of specific circRNAs was observed. Particularly, we demonstrated that strong upregulation of circRNAs processed from the zinc finger E\u2010box binding homeobox 1 (<jats:italic>ZEB1<\/jats:italic>) transcription factor; this upregulation was exclusive to <jats:italic>SF3B1<\/jats:italic>\u2010mutated patients and was not observed in those with mutations in other splicing factors or other recurrently mutated genes, or with other clinical variables. Furthermore, we focused on the most upregulated <jats:italic>ZEB1<\/jats:italic>\u2010circRNA, hsa_circ_0000228, and, by its knockdown, we demonstrated that its expression is related to mitochondrial activity. Using microRNA analyses,\u00a0we\u00a0proposed miR\u20101248 as a direct target of hsa_circ_0000228. To conclude, we demonstrated that mutated <jats:italic>SF3B1<\/jats:italic> leads to deregulation of <jats:italic>ZEB1<\/jats:italic>\u2010circRNAs, potentially contributing to the defects in mitochondrial metabolism observed in <jats:italic>SF3B1<\/jats:italic>\u2010mutated MDS.<\/jats:p>","DOI":"10.1002\/1878-0261.13486","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T07:29:42Z","timestamp":1688628582000},"page":"2565-2583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Expression of circular <scp>RNAs<\/scp> in myelodysplastic neoplasms and their association with mutations in the splicing factor gene <i>SF3B1<\/i>"],"prefix":"10.1002","volume":"17","author":[{"given":"Iva","family":"Trsova","sequence":"first","affiliation":[{"name":"Department of Genomics Institute of Hematology and Blood Transfusion  Prague Czech Republic"},{"name":"Department of Genetics and 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