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Oncolytic virotherapy has emerged as a new treatment modality which uses tumour\u2010specific viruses to eliminate cancerous cells. Non\u2010human primate adenoviruses of the human adenovirus B (HAdV\u2010B) species have demonstrated considerable lytic potential in human\u00a0cancer cells as well as limited preexisting neutralizing immunity in humans. Previously, we have generated a new oncolytic derivative of the gorilla\u2010derived HAdV\u2010B AdV\u2010lumc007 named \u2018GoraVir\u2019. Here, we show that GoraVir displays oncolytic efficacy in pancreatic cancer cells and pancreatic\u2010cancer\u2010associated fibroblasts. Moreover, it retains its lytic potential in monoculture and co\u2010culture spheroids. In addition, we established the ubiquitously expressed complement receptor CD46 as the main entry receptor for GoraVir. Finally, a single intratumoural dose of GoraVir was shown to delay tumour growth in a BxPC\u20103 xenograft model at 10\u2009days post\u2010treatment. Collectively, these data demonstrate that the new gorilla\u2010derived oncolytic adenovirus is a potent oncolytic vector candidate that targets both pancreatic cancer cells and tumour\u2010adjacent stroma.<\/jats:p>","DOI":"10.1002\/1878-0261.13561","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T10:12:59Z","timestamp":1701425579000},"page":"1245-1258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Preclinical evaluation of the gorilla\u2010derived <scp>HAdV\u2010B AdV<\/scp>\u2010lumc007 oncolytic adenovirus \u2018<scp>GoraVir<\/scp>\u2019 for the treatment of pancreatic ductal adenocarcinoma"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1844-9247","authenticated-orcid":false,"given":"Selas T. 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