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However, owing to the previously reported tumorigenic potential of NSC cell lines after intranasal administration (INA), here we identified the human hepatic stellate cell line LX\u20102 as a cell type capable of longer resistance to replication of oncolytic adenoviruses (OAVs) as a therapeutic cargo, and that is non\u2010tumorigenic after INA. Our data show that LX\u20102 cells can longer withstand the OAV XVir\u2010N\u201031 replication and oncolysis than NSCs. By selecting the highly migratory cell population out of LX\u20102, an offspring cell line with a higher and more stable capability to migrate was generated. Additionally, as a safety backup, we applied genomic herpes simplex virus thymidine kinase (HSV\u2010TK) integration into LX\u20102, leading to high vulnerability to ganciclovir (GCV). Histopathological analyses confirmed the absence of neoplasia in the respiratory tracts and brains of immuno\u2010compromised mice 3\u2009months after INA of LX\u20102 cells. 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