{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:26:39Z","timestamp":1792175199922,"version":"4.4.0"},"reference-count":73,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001592","name":"Higher Education Authority","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001592","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Circulating tumor cells (CTCs) have potential as diagnostic, prognostic, and predictive biomarkers in solid tumors. Despite Food and Drug Administration (FDA) approval of CTC devices in various cancers, the rarity and heterogeneity of CTCs in lung cancer make them technically challenging to isolate and analyze, hindering their clinical integration. Establishing a consensus through comparative analysis of different CTC systems is warranted. This study aimed to evaluate seven different CTC enrichment methods across five technologies using a standardized spike\u2010in protocol: the CellMag\u2122 (EpCAM\u2010dependent enrichment), EasySep\u2122 and RosetteSep\u2122 (blood cell depletion), and the Parsortix\u00ae PR1 and the new design Parsortix\u00ae Prototype (PP) (size\u2010 and deformability\u2010based enrichment). The Parsortix\u00ae systems were also evaluated for any differences in recovery rates between cell harvest versus in\u2010cassette staining. Healthy donor blood (5\u2009mL) was spiked with 100 fluorescently labeled EpCAM\n                    <jats:sup>high<\/jats:sup>\n                    H1975 cells, processed through each system, and the isolation efficiency was calculated. The CellMag\u2122 had the highest recovery rate (70\u2009\u00b1\u200914%), followed by Parsortix\u00ae PR1 in\u2010cassette staining, while the EasySep\u2122 had the lowest recovery (18\u2009\u00b1\u20098%). Additional spike\u2010in experiments were performed with EpCAM\n                    <jats:sup>moderate<\/jats:sup>\n                    A549 and EpCAM\n                    <jats:sup>low<\/jats:sup>\n                    H1299 cells using the CellMag\u2122 and Parsortix\u00ae PR1 in\u2010cassette staining. The recovery rate of CellMag\u2122 significantly reduced to 35\u2009\u00b1\u200914% with A549 cells and 1\u2009\u00b1\u20091% with H1299 cells. However, the Parsortix\u00ae PR1 in\u2010cassette staining showed cell phenotype\u2010independent and consistent recovery rates among all lung cancer cell lines: H1975 (49\u2009\u00b1\u20092%), A549 (47\u2009\u00b1\u200910%), and H1299 (52\u2009\u00b1\u200910%). Furthermore, we demonstrated that the Parsortix\u00ae PR1 in\u2010cassette staining method is capable of isolating heterogeneous single CTCs and cell clusters from patient samples. The Parsortix\u00ae PR1 in\u2010cassette staining, capable of isolating different phenotypes of CTCs as either single cells or cell clusters with consistent recovery rates, is considered optimal for CTC enrichment for lung cancer, albeit needing further optimization and validation.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13705","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T06:06:20Z","timestamp":1722924380000},"page":"2014-2037","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer"],"prefix":"10.1002","volume":"19","author":[{"given":"Volga","family":"M Saini","sequence":"first","affiliation":[{"name":"Thoracic Oncology Research Group, Trinity Translational Medicine Institute St James's Hospital  Dublin Ireland"},{"name":"Department of Clinical Medicine, School of Medicine Trinity College Dublin  Ireland"},{"name":"Trinity St. James's Cancer Institute Trinity College Dublin  Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4684-8221","authenticated-orcid":false,"given":"Ezgi","family":"Oner","sequence":"additional","affiliation":[{"name":"Thoracic Oncology Research Group, Trinity Translational Medicine Institute St James's Hospital  Dublin Ireland"},{"name":"Department of Clinical Medicine, School of Medicine Trinity College Dublin  Ireland"},{"name":"Trinity St. James's Cancer Institute Trinity College Dublin  Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"Ward","sequence":"additional","affiliation":[{"name":"Trinity St. James's Cancer Institute Trinity College Dublin  Ireland"},{"name":"Department of Obstetrics and Gynaecology, School of Medicine Trinity College Dublin  Ireland"},{"name":"Department of Histopathology and Morbid Anatomy, School 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J.","family":"Fitzmaurice","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery St James's Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"O'Leary","sequence":"additional","affiliation":[{"name":"Trinity St. James's Cancer Institute Trinity College Dublin  Ireland"},{"name":"Department of Histopathology and Morbid Anatomy, School of Medicine Trinity College Dublin  Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"O'Toole","sequence":"additional","affiliation":[{"name":"Trinity St. James's Cancer Institute Trinity College Dublin  Ireland"},{"name":"Department of Obstetrics and Gynaecology, School of Medicine Trinity College Dublin  Ireland"},{"name":"Department of Histopathology and Morbid Anatomy, School of Medicine Trinity College Dublin  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