{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:28:44Z","timestamp":1790692124381,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000969","name":"Australian Academy of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000969","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>\n                    The emergence of targeted therapies has transformed ovarian cancer treatment. However, biomarker profiling for precision medicine is limited by access to quality, tumour\u2010enriched tissue samples. The use of cell\u2010free DNA (cfDNA) in ascites presents a potential solution to this challenge. In this study, next\u2010generation sequencing was performed on ascites\u2010derived cfDNA samples (26 samples from 15 human participants with ovarian cancer), with matched DNA from ascites\u2010derived tumour cells (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) and archived formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095). Similar tumour purity and variant detection were achieved with cfDNA compared to FFPE and ascites cell DNA. Analysis of large\u2010scale genomic alterations, loss of heterozygosity and tumour mutation burden identified six cases of high genomic instability (including four with pathogenic\n                    <jats:italic>BRCA1<\/jats:italic>\n                    and\n                    <jats:italic>BRCA2<\/jats:italic>\n                    mutations). Copy number profiles and subclone prevalence changed between sequential ascites samples, particularly in a case where deletions and chromothripsis in Chr17p13.1 and Chr8q resulted in changes in clinically relevant\n                    <jats:italic>TP53<\/jats:italic>\n                    and\n                    <jats:italic>MYC<\/jats:italic>\n                    variants over time. Ascites cfDNA identified clinically actionable information, concordant to tissue biopsies, enabling opportunistic molecular profiling. This advocates for analysis of ascites cfDNA\n                    <jats:italic>in lieu<\/jats:italic>\n                    of accessing tumour tissue via biopsy.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13710","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T07:44:38Z","timestamp":1723103078000},"page":"2668-2683","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Cell\u2010free\n                    <scp>DNA<\/scp>\n                    from ascites identifies clinically relevant variants and tumour evolution in patients with advanced ovarian cancer"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5208-1613","authenticated-orcid":false,"given":"Bonnita","family":"Werner","sequence":"first","affiliation":[{"name":"Gynaecological Cancer Research Group, School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elyse","family":"Powell","sequence":"additional","affiliation":[{"name":"Gynaecological Cancer Research Group, School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Duggan","sequence":"additional","affiliation":[{"name":"Gynaecological Oncology Department Royal Hospital for Women  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilisa","family":"Cortesi","sequence":"additional","affiliation":[{"name":"Gynaecological Cancer Research Group, School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"},{"name":"Laboratory of Cellular and Molecular Engineering, Department of Electrical, Electronic and Information Engineering Alma Mater Studiorum\u2010University of Bologna  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeh Chen","family":"Lee","sequence":"additional","affiliation":[{"name":"Gynaecological Oncology Department Royal Hospital for Women  Sydney Australia"},{"name":"School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Arora","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"},{"name":"Prince of Wales Private Hospital  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramanand","family":"Athavale","sequence":"additional","affiliation":[{"name":"Gynaecological Oncology Department Royal Hospital for Women  Sydney Australia"},{"name":"School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2234-0631","authenticated-orcid":false,"given":"Michael","family":"Dean","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Genomics, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Warton","sequence":"additional","affiliation":[{"name":"Gynaecological Cancer Research Group, School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4438-2309","authenticated-orcid":false,"given":"Caroline E.","family":"Ford","sequence":"additional","affiliation":[{"name":"Gynaecological Cancer Research Group, School of Clinical Medicine, Faculty of Medicine and Health University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22935"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2023.07.011"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1902626"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000199"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2013_071"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.14682"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404\u2010020\u201005553\u2010y"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001534"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.01.054"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2023.189052"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2021.06.028"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010023\u201002424\u20106"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201004\u20101529"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.4143\/crt.2019.700"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010023\u201001864\u20101"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40364\u2010023\u201000533\u20101"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/ijgc\u20102021\u2010002978"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0908806"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1706174"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2015.05.004"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090\u20108258(22)01365\u20108"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010023\u201001320\u20102"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14410"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001091"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010018\u20100531\u20108"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa420"},{"key":"e_1_2_11_28_1","unstructured":"UNSW Sydney.Katana.2010."},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17157"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004873"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa261"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010020\u201003919\u20102"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10166"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(17)32440\u20106"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2023.06.467"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12113468"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1200\/PO.22.00355"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13102386"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11081044"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101055"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T13:23:37Z","timestamp":1731072217000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.13710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,8]]},"references-count":39,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/1878-0261.13710"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.13710","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13710\/v3\/review1","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.03.09.24303840","asserted-by":"object"}]},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,8]]},"assertion":[{"value":"2024-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}