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Recent research has shown that microRNA 200c (miR\u2010200c) can hamper certain steps of the invasion\u2013metastasis cascade. However, it is still unclear whether miR\u2010200c expression alone is sufficient to prevent breast cancer cells from metastasis formation. Hence, we performed a xenograft mouse experiment with inducible miR\u2010200c expression in MDA\u2010MB 231 cells. The <jats:italic>ex vivo<\/jats:italic> analysis of metastatic sites in a multitude of organs, including lung, liver, brain, and spleen, revealed a dramatically reduced metastatic burden in mice with miR\u2010200c\u2010expressing tumors. A fundamental prerequisite for metastasis formation is the motility of cancer cells and, therefore, their migration. Consequently, we analyzed the effect of miR\u2010200c on collective\u2010 and single\u2010cell migration <jats:italic>in vitro<\/jats:italic>, utilizing MDA\u2010MB 231 and MCF7 cell systems with genetically modified miR\u2010200c expression. Analysis of collective\u2010cell migration revealed confluence\u2010dependent motility of cells with altered miR\u2010200c expression. Additionally, scratch assays showed an enhanced predisposition of miR\u2010200c\u2010negative cells to leave cell clusters. The in\u2010between stage of collective\u2010 and single\u2010cell migration was validated using transwell assays, which showed reduced migration of miR\u2010200c\u2010positive cells. Finally, to measure migration at the single\u2010cell level, a novel assay on dumbbell\u2010shaped micropatterns was performed, which revealed that miR\u2010200c critically determines confined cell motility. All of these results demonstrate that sole expression of miR\u2010200c impedes metastasis formation <jats:italic>in vivo<\/jats:italic> and migration <jats:italic>in vitro<\/jats:italic> and highlights miR\u2010200c as a metastasis suppressor in breast cancer.<\/jats:p>","DOI":"10.1002\/1878-0261.13712","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T12:27:39Z","timestamp":1728995259000},"page":"1029-1053","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unraveling the metastasis\u2010preventing effect of <scp>miR<\/scp>\u2010200c <i>in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1002","volume":"19","author":[{"given":"Bianca","family":"K\u00f6hler","sequence":"first","affiliation":[{"name":"Pharmaceutical Biotechnology, Department of Pharmacy Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  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