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Due to a lack of an early detection test\u00a0and overt symptoms, many patients are diagnosed at a late stage where metastasis makes treatment very challenging. Furthermore, the current standard treatment for HGSOC patients, consisting of debulking surgery and platinum\u2010taxane chemotherapy, reduces quality of life due to debilitating side\u2010effects. Sadly, 80\u201390% of patients diagnosed with advanced stage ovarian cancer will die due to treatment resistance. As such, novel therapeutic strategies for HGSOC that are both more effective and less toxic are urgently required. Here we describe the assessment of cold atmospheric pressure (CAP) gas discharge technology as a novel treatment strategy in pre\u2010clinical models of HGSOC. Plasma\u2010activated media (PAM) was generated using cell growth media. HGSOC cell lines, patient ascites cells and primary tissue explants were tested for their response to PAM via analysis of cell viability, cell death and oxidative stress assays. Our data show that PAM treatment can be more effective than standard carboplatin chemotherapy at selectively targeting ovarian cancer cells in primary patient samples. Further, we also observed PAM to induce apoptosis in HGSOC cancer cell lines via induction of oxidative stress and mitochondrial\u2010mediated apoptosis. These findings suggest that PAM is a viable therapeutic strategy to test in\n                    <jats:italic>in vivo<\/jats:italic>\n                    models of ovarian cancer, with a view to develop an intraperitoneal PAM\u2010based therapy for HGSOC patients. Our studies validate the ability of PAM to selectively target tumour tissue and ascites cells. This work supports the development of PAM towards\n                    <jats:italic>in vivo<\/jats:italic>\n                    validation and translation into clinical practice.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13768","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T19:44:22Z","timestamp":1733255062000},"page":"1170-1187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasma\u2010activated media selectively induces apoptotic death via an orchestrated oxidative stress pathway in high\u2010grade serous ovarian cancer cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Lorena T.","family":"Davies","sequence":"first","affiliation":[{"name":"Centre for Cancer Biology University of South Australia and SA Pathology  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja","family":"Ganesen","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology University of South Australia and SA Pathology  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Toubia","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology University of South Australia and SA Pathology  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Ha","family":"Hong","sequence":"additional","affiliation":[{"name":"Future Industries Institute University of South Australia  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushil","family":"Kumar KC","sequence":"additional","affiliation":[{"name":"Future Industries Institute University of South Australia  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin K.","family":"Oehler","sequence":"additional","affiliation":[{"name":"Reproductive Cancer Research Group; Discipline of Obstetrics and Gynaecology, Adelaide Medical School The University of Adelaide  Australia"},{"name":"Robinson Research Institute  Adelaide Australia"},{"name":"Department of Gynaecological Oncology Royal Adelaide Hospital  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Ricciardelli","sequence":"additional","affiliation":[{"name":"Reproductive Cancer Research Group; Discipline of Obstetrics and Gynaecology, Adelaide Medical School The University of Adelaide  Australia"},{"name":"Robinson Research Institute  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endre J.","family":"Szili","sequence":"additional","affiliation":[{"name":"Future Industries Institute University of South Australia  Adelaide Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirmal","family":"Robinson","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology University of South Australia and SA Pathology  Adelaide Australia"},{"name":"Adelaide Medical School The University of Adelaide  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9587-3837","authenticated-orcid":false,"given":"Melissa R.","family":"Pitman","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology University of South Australia and SA Pathology  Adelaide Australia"},{"name":"Robinson Research Institute  Adelaide Australia"},{"name":"School of Biological Sciences The University of Adelaide  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Institute NC.SEER*Explorer: an interactive website for SEER cancer statistics [Internet].2023Surveillance Research Program National Cancer Institute National Cancer Institute SEER Incidence Data November 2022 Submission (1975\u20132020) SEER 22 registries (excluding Illinois and Massachusetts). Expected Survival Life Tables by Socio\u2010Economic Standards."},{"key":"e_1_2_11_3_1","volume-title":"Cancer Data in Australia","author":"Welfare 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