{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:05:23Z","timestamp":1777100723288,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:p>Male infertility is a risk factor for the development of testicular germ cell tumors. In this study, we investigated microRNA profiles in seminal plasma to identify potential noninvasive biomarkers able to discriminate the men at highest risk of developing cancer among the infertile population. We compared the microRNA profiles of individuals affected by testicular germ cell tumors and healthy individuals with normal or impaired spermiograms using high\u2010throughput technology and confirmed the results by single\u2010assay digital PCR. We found that miR\u2010221\u20103p and miR\u2010222\u20103p were downregulated and miR\u2010126\u20103p was upregulated in cancer patients compared to both infertile and fertile men. ROC curve analysis confirmed that miR\u2010126 upregulation is able to identify cancer patients among the infertile male population. In addition, in\u2010depth bioinformatics analysis based on weighted gene co\u2010expression networks showed that the identified miRNAs regulate cellular pathways involved in cancer.<\/jats:p>","DOI":"10.1002\/1878-0261.13784","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T18:23:55Z","timestamp":1734373435000},"page":"1188-1202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>M<\/scp>icro<scp>RNA<\/scp>s in seminal plasma are able to discern infertile men at increased risk of developing testicular cancer"],"prefix":"10.1002","volume":"19","author":[{"given":"Carmen","family":"Ferrara","sequence":"first","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4868-3045","authenticated-orcid":false,"given":"Rosalia","family":"Battaglia","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Caponnetto","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Fazzio","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Stella","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Barbagallo","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicol\u00f2","family":"Musso","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University of Enna \"Kore\"  Enna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Lunelio","sequence":"additional","affiliation":[{"name":"IVF Unit Cannizzaro Hospital  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Elena","family":"Vento","sequence":"additional","affiliation":[{"name":"IVF Unit Cannizzaro Hospital  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Placido","family":"Borz\u00ec","sequence":"additional","affiliation":[{"name":"IVF Unit Cannizzaro Hospital  Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Scollo","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Division, Maternal and Child Department, Cannizzaro Hospital Catania Kore University of Enna  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Barbagallo","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Ragusa","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Pernagallo","sequence":"additional","affiliation":[{"name":"DESTINA Genomica S.L.  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinzia","family":"Di Pietro","sequence":"additional","affiliation":[{"name":"Section of Biology and Genetics \u201cG. Sichel\u201d, Department of Biomedical and Biotechnological Sciences University of Catania  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00032"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgab523"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.08.002"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010018\u20100029\u20100"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina59071305"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.11.005"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2009.179"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140\u20106736(92)92425\u2010f"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.26.4481"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna6010002"},{"key":"e_1_2_11_12_1","first-page":"113","article-title":"Biomarkers in testicular cancer","volume":"75","author":"Mayor\u2010de\u2010Castro J","year":"2022","journal-title":"Arch Esp Urol"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911502"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/life11080736"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.708765"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2023.0314"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14\u2010259382"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900587\u2010MCP200"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024821"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det099"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.169714"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020\u2010016\u20101150\u2010z"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.17305\/bjbms.2018.3477"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1079"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010019\u20103105\u2010x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physrep.2019.12.004"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes14071375"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15153944"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20108900\u20103_1"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/925350"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652412798376170"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7252013"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2022.02.005"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428\u2010012\u20101325\u20109"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2017.00066"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201016\u20100714"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2023.104159"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.087403"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.07.008"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232213676"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2015.549"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016617"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.02.021"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024950"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.08.036"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.2147\/JBM.S347397"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15396"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010014\u20100259\u20101"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154\u2010015\u20109322\u20103"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020\u2010023\u201003375\u2010x"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201041915\u20107"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13784","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T17:48:56Z","timestamp":1744134536000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.13784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,16]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/1878-0261.13784"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.13784","archive":["Portico"],"relation":{},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,16]]},"assertion":[{"value":"2024-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}