{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:32:30Z","timestamp":1789428750804,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1S77920N"],"award-info":[{"award-number":["1S77920N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G009324N"],"award-info":[{"award-number":["G009324N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G094219N"],"award-info":[{"award-number":["G094219N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1S26125N"],"award-info":[{"award-number":["1S26125N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["11PFT24N"],"award-info":[{"award-number":["11PFT24N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018675","name":"Kids' Cancer Project","doi-asserted-by":"publisher","award":["APP1188234"],"award-info":[{"award-number":["APP1188234"]}],"id":[{"id":"10.13039\/501100018675","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001111","name":"Cancer Australia","doi-asserted-by":"publisher","award":["APP1188234"],"award-info":[{"award-number":["APP1188234"]}],"id":[{"id":"10.13039\/501100001111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:p>\n                    High\u2010risk neuroblastomas, often associated with\n                    <jats:italic>MYCN<\/jats:italic>\n                    protooncogene amplification, are addicted to polyamines, small polycations vital for cellular functioning. We have previously shown that neuroblastoma cells increase polyamine uptake when exposed to the polyamine biosynthesis inhibitor difluoromethylornithine (DFMO), and this mechanism is thought to limit the efficacy of the drug in clinical trials. This finding resulted in the clinical development of polyamine transport inhibitors, including AMXT 1501, which is presently under clinical investigation in combination with DFMO. However, the mechanisms and transporters involved in DFMO\u2010induced polyamine uptake are unknown. Here, we report that knockdown of ATPase 13A3 (\n                    <jats:italic>ATP13A3<\/jats:italic>\n                    ), a member of the P5B\u2010ATPase polyamine transporter family, limited basal and DFMO\u2010induced polyamine uptake, attenuated\n                    <jats:italic>MYCN<\/jats:italic>\n                    \u2010amplified and non\u2010\n                    <jats:italic>MYCN<\/jats:italic>\n                    \u2010amplified neuroblastoma cell growth, and potentiated the inhibitory effects of DFMO. Conversely, overexpression of ATP13A3 in neuroblastoma cells increased polyamine uptake, which was inhibited by AMXT 1501, highlighting ATP13A3 as a key target of the drug. An association between high\n                    <jats:italic>ATP13A3<\/jats:italic>\n                    expression and poor survival in neuroblastoma further supports a role of this transporter in neuroblastoma progression. Thus, this study identified ATP13A3 as a critical regulator of basal and DFMO\u2010induced polyamine uptake and a novel therapeutic target for neuroblastoma.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13789","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T06:46:51Z","timestamp":1740120411000},"page":"913-936","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The polyamine transporter\n                    <scp>ATP13A3<\/scp>\n                    mediates difluoromethylornithine\u2010induced polyamine uptake in neuroblastoma"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5822-3969","authenticated-orcid":false,"given":"Mujahid","family":"Azfar","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine KU Leuven  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiman","family":"Gao","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6650-3845","authenticated-orcid":false,"given":"Chris","family":"Van den Haute","sequence":"additional","affiliation":[{"name":"Group for Neurobiology and Gene Therapy KU Leuven  Belgium"},{"name":"Leuven Viral Vector Core KU Leuven  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9682-1118","authenticated-orcid":false,"given":"Mawar","family":"Karsa","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruby","family":"Pandher","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayu","family":"Karsa","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4079-3801","authenticated-orcid":false,"given":"Dayna","family":"Spurling","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1211-2440","authenticated-orcid":false,"given":"Emma","family":"Ronca","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Bongers","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Guo","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6398-3046","authenticated-orcid":false,"given":"Chelsea","family":"Mayoh","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0031-7713","authenticated-orcid":false,"given":"Youri","family":"Fayt","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine KU Leuven  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1690-6324","authenticated-orcid":false,"given":"Arthur","family":"Schoofs","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine KU Leuven  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Burns","sequence":"additional","affiliation":[{"name":"Aminex Therapeutics Aminex Therapeutics Inc.  Kirkland WA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7400-1319","authenticated-orcid":false,"given":"Steven H. L.","family":"Verhelst","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Biology, Department of Cellular and Molecular Medicine KU Leuven  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0632-4589","authenticated-orcid":false,"given":"Murray D.","family":"Norris","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"},{"name":"UNSW Centre for Childhood Cancer Research UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2036-8817","authenticated-orcid":false,"given":"Michelle","family":"Haber","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7822-2944","authenticated-orcid":false,"given":"Peter","family":"Vangheluwe","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine KU Leuven  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7644-4121","authenticated-orcid":false,"given":"Klaartje","family":"Somers","sequence":"additional","affiliation":[{"name":"Children's Cancer Institute, Lowy Cancer Research Centre UNSW Sydney  Australia"},{"name":"School of Clinical Medicine, UNSW Medicine &amp; Health UNSW Sydney  Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21750"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010022\u201000643\u2010z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.59.4648"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-018-0050-3"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20107398\u20109_39"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25074"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33044"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2022.40.16_suppl.10010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201032659\u2010w"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aau1099"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28139"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-20896-z"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1968-7"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.013908"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2022.119354"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07712-4"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13060918"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508220112"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4569"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201028648\u20108"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13020337"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/1087057114521867"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw230"},{"issue":"1","key":"e_1_2_11_25_1","first-page":"215","article-title":"The gastrointestinal tract as polyamine source for tumor growth","volume":"9","author":"Sarhan S","year":"1989","journal-title":"Anticancer Res"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127246"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm801580w"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-13-0120-T"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007750050321"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.624079"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2012.00162"},{"issue":"6","key":"e_1_2_11_32_1","first-page":"1231","article-title":"ATP13A3 and caveolin\u20101 as potential biomarkers for difluoromethylornithine\u2010based therapies in pancreatic cancers","volume":"6","author":"Madan M","year":"2016","journal-title":"Am J Cancer Res"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvae068"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M804714200"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308325200"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1251100"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22006"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2020.105035"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.18568"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251\u2010021\u201001216\u20107"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578\u2010020\u201000506\u2010z"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11605-021-05099-7"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2020.11700"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12957\u2010015\u20100646\u2010y"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922342117"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03672-4"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/bse0460004"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0350318"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209521"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-11-0178"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010021\u201000367\u20109"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541\u20107786.Mcr\u201013\u20100536"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a014415"}],"updated-by":[{"DOI":"10.1002\/1878-0261.70088","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000}}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.13789","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T01:19:58Z","timestamp":1741569598000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.13789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,21]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/1878-0261.13789"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.13789","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.13789\/v1\/decision1","asserted-by":"object"}]},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,21]]},"assertion":[{"value":"2024-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}