{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:57:14Z","timestamp":1792508234644,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["NKI2014\u20107080"],"award-info":[{"award-number":["NKI2014\u20107080"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n                    Cell\u2010free circulating tumor DNA (ctDNA) has emerged as a promising biomarker for response evaluation in metastatic castration\u2010resistant prostate cancer (mCRPC). The current study evaluated the modified fast aneuploidy screening test\u2010sequencing system (mFast\u2010SeqS), a quick, tumor\u2010agnostic and affordable ctDNA assay that requires a small input of DNA, to generate a genome\u2010wide aneuploidy (GWA) score in mCRPC patients, and correlated this to matched metastatic tumor biopsies. In this prospective multicenter study, GWA scores were evaluated from blood samples of 196 mCRPC patients prior to treatment (baseline) with taxanes (docetaxel and cabazitaxel) and androgen receptor signaling inhibitors (ARSI; abiraterone and enzalutamide), and from 74 mCRPC patients at an early timepoint during treatment (early timepoint; median 21\u2009days).\n                    <jats:italic>Z<\/jats:italic>\n                    \u2010scores per chromosome arm were tested for their association with tumor tissue genomic alterations. We found that a high tumor load in blood (GWA\n                    <jats:sup>high<\/jats:sup>\n                    ) at baseline was associated with poor response to ARSI [HR: 2.63 (95% CI: 1.86\u20133.72)\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001] but not to taxanes. Interestingly, GWA\n                    <jats:sup>high<\/jats:sup>\n                    score at the early timepoint was associated with poor response to both ARSIs [HR: 6.73 (95% CI: 2.60\u201317.42)\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001] and taxanes [2.79 (95% CI: 1.34\u20135.78)\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.006]. A significant interaction in Cox proportional hazards analyses was seen when combining GWA status and type of treatment (at baseline\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.008; early timepoint\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.018). In summary, detection of ctDNA in blood by mFast\u2010SeqS is cheap, fast and feasible, and could be used at different timepoints as a potential predictor for outcome to ARSI and taxane treatment in mCRPC.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13797","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T05:39:20Z","timestamp":1741930760000},"page":"2822-2832","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cell\u2010free\n                    <scp>DNA<\/scp>\n                    aneuploidy score as a dynamic early response marker in prostate cancer"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3006-5206","authenticated-orcid":false,"given":"Khrystany T.","family":"Isebia","sequence":"first","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7381-3171","authenticated-orcid":false,"given":"Anouk C.","family":"de Jong","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisanne F.","family":"van Dessel","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanja","family":"de Weerd","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corine","family":"Beaufort","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Helmijr","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9153-4280","authenticated-orcid":false,"given":"Jos\u00e9 Alberto","family":"Nakauma\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Urology, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Job","family":"van Riet","sequence":"additional","affiliation":[{"name":"Division of AI in Oncology German Cancer Research Center (DFKZ)  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Hamberg","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Franciscus Gasthuis &amp; Vlietland  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Vis","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology The Netherlands Cancer Institute\u2010Antoni van Leeuwenhoek Hospital  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel S.","family":"van der Heijden","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology The Netherlands Cancer Institute\u2010Antoni van Leeuwenhoek Hospital  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Beije","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martijn P.","family":"Lolkema","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3055-3066","authenticated-orcid":false,"given":"Teoman","family":"Deger","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia M.","family":"Wilting","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"de Wit","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1258-9804","authenticated-orcid":false,"given":"Maurice P. H. M.","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology and Cancer Genomics Netherlands, Erasmus MC Cancer Institute University Medical Center Rotterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3428-3366","authenticated-orcid":false,"given":"John W. 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