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Mutations in this gene are implicated in syndromes involving premature ovarian failure and granulosa cell tumors (GCTs). This rare cancer accounts for less than 5% of diagnosed ovarian cancers and is causally associated with the\n                    <jats:italic>FOXL2<\/jats:italic>\n                    c.402C&gt;G, p.C134W mutation in 97% of the adult cases (AGCTs). In this study, we employed CRISPR technology to specifically eliminate the\n                    <jats:italic>FOXL2<\/jats:italic>\n                    c.402C&gt;G mutation in granulosa tumor cells. Our results show that this Cas9\u2010mediated strategy selectively targets the mutation without affecting the wild\u2010type allele. Granulosa cells lacking\n                    <jats:italic>FOXL2<\/jats:italic>\n                    c.402C&gt;G exhibit a reduced malignant phenotype, with significant changes in cell proliferation and invasion. Furthermore, these modified cells are more susceptible to dasatinib and ketoconazole. Transcriptomic and proteomic analyses reveal that CRISPR\u2010modified granulosa tumor cells shift their expression profiles towards a wild\u2010type\u2010like phenotype. Additionally, this altered expression signature has led to the identification of new compounds with antiproliferative and pro\u2010apoptotic effects on granulosa tumor cells. Our findings demonstrate the potential of CRISPR technology for the specific targeting and elimination of a mutation causing GCTs, highlighting its therapeutic promise for treating this rare ovarian cancer.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13799","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T10:58:55Z","timestamp":1736333935000},"page":"1092-1116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CRISPR<\/scp>\n                    targeting of\n                    <scp>\n                      <i>FOXL2<\/i>\n                    <\/scp>\n                    c.\n                    <scp>402C<\/scp>\n                    &gt;G mutation reduces malignant phenotype in granulosa tumor cells and identifies anti\u2010tumoral 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