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Studies using tumor\u2010agnostic approaches report ctDNA in 10\u201315% of patients, though with uncertainty as to whether the signal originates from the adenoma. To obtain an accurate estimate of the proportion of patients with ctDNA, a sensitive tumor\u2010informed strategy is preferred, as it ensures the detected signal originates from the adenoma. Here, tumor\u2010informed whole\u2010genome sequencing\u2010based ctDNA analysis (MRD\u2010EDGE\n                    <jats:sup>SNV<\/jats:sup>\n                    ) was applied to two independent cohorts. Cohort 1, comprising 93 patients with stage III colorectal cancer (CRC) and 40 healthy individuals, was used to establish the signal threshold at 95% specificity. This threshold was then applied to Cohort 2, consisting of 22 patients with symptomatic and 20 with asymptomatic adenomas. In stage III, MRD\u2010EDGE\n                    <jats:sup>SNV<\/jats:sup>\n                    had an area under the curve of 0.98. ctDNA was detected in 50% and 25% of patients with symptomatic and asymptomatic adenomas, respectively. The median adenoma plasma tumor fraction was 5.9\u2009\u00d7\u200910\n                    <jats:sup>\u22125<\/jats:sup>\n                    . These finding not only demonstrate the feasibility of ctDNA detection in patients with colorectal adenomas, but also provides an estimate of the necessary sensitivity required to detect these lesions, paving the way for future ctDNA\u2010based screening strategies.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.13803","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T06:45:21Z","timestamp":1737355521000},"page":"984-993","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Whole\u2010genome sequencing of cell\u2010free\n                    <scp>DNA<\/scp>\n                    reveals\n                    <scp>DNA<\/scp>\n                    of tumor origin in plasma from patients with colorectal adenomas"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9552-5421","authenticated-orcid":false,"given":"Amanda","family":"Frydendahl","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Widman","sequence":"additional","affiliation":[{"name":"Memorial Sloan Kettering Cancer Center  New York NY USA"},{"name":"New York Genome Center  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"\u00d8gaard","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anushri","family":"Arora","sequence":"additional","affiliation":[{"name":"New York Genome Center  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Halmos","sequence":"additional","affiliation":[{"name":"New York Genome Center  New York NY USA"},{"name":"Weill Cornell Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper","family":"Nors","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9175-0373","authenticated-orcid":false,"given":"Johanne","family":"Ahrenfeldt","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tenna V.","family":"Henriksen","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Demuth","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Line","family":"Raaby","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"},{"name":"Department of Pathology Aarhus University Hospital  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads H.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Therkildsen","sequence":"additional","affiliation":[{"name":"Gastro Unit, Copenhagen University Hospital Amager \u2013 Hvidovre Hospital  Hvidovre Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan A.","family":"Landau","sequence":"additional","affiliation":[{"name":"New York Genome Center  New York NY USA"},{"name":"Weill Cornell Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7406-2103","authenticated-orcid":false,"given":"Claus L.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of 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