{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:15:16Z","timestamp":1787238916414,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020581","name":"Fondazione AIRC per la ricerca sul cancro ETS","doi-asserted-by":"publisher","award":["23443"],"award-info":[{"award-number":["23443"]}],"id":[{"id":"10.13039\/100020581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020581","name":"Fondazione AIRC per la ricerca sul cancro ETS","doi-asserted-by":"publisher","award":["23520"],"award-info":[{"award-number":["23520"]}],"id":[{"id":"10.13039\/100020581","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:p>Despite many efforts to understand the molecular mechanisms of pancreatic ductal adenocarcinoma (PDAC) treatment resistance, there is still no reliable method for selecting patients who could benefit from standard pharmacological treatment. Here, four PDAC patient\u2010derived xenografts (PDAC\u2010PDXs) with different responses to gemcitabine plus nab\u2010paclitaxel (nanoparticle albumin\u2010bound paclitaxel) were studied to dissect the contribution of both tumor and host microenvironment to treatment response. PDAC\u2010PDXs transplanted into the pancreas of immunodeficient mice retained the main genetic and histopathological characteristics of the original human tumors, including invasiveness and desmoplastic reaction. Response to chemotherapy was associated with a specific 294 stroma gene signature and was not due to the intrinsic responsiveness of tumor cells or differences in drug delivery. Human dataset analysis validated the expression of the 294 stroma gene signature in PDAC clinical samples, confirming PDAC\u2010PDXs as a useful tool to study the biology of tumor\u2013host interactions and to test drug efficacy. In summary, we identified a stroma gene signature that differentiates PDAC\u2010PDXs that are responsive to gemcitabine plus Nab\u2010paclitaxel treatment from those that are not, confirming the active role of the tumor microenvironment in the drug response.<\/jats:p>","DOI":"10.1002\/1878-0261.13816","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T04:24:37Z","timestamp":1738643077000},"page":"1075-1091","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Stroma gene signature predicts responsiveness to chemotherapy in pancreatic ductal adenocarcinoma patient\u2010derived xenograft models"],"prefix":"10.1002","volume":"19","author":[{"given":"Alessia","family":"Anastasia","sequence":"first","affiliation":[{"name":"Department of Oncology Istituto di Ricerche Farmacologiche Mario Negri IRCCS  Bergamo and Milan 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