{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:16:41Z","timestamp":1789874201091,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:p>\n                    Genetic testing in epithelial ovarian cancer (EOC) in Ontario includes germline next\u2010generation sequencing (NGS) for 19 genes. Additionally, tumor tissue undergoes reflex NGS testing for\n                    <jats:italic>BRCA1\/2<\/jats:italic>\n                    to assess eligibility for PARPi. Although parallel testing confers advantages, this model duplicates healthcare resources. Here, we prospectively assessed the feasibility of tumor\u2010first multigene testing by comparing tumor tissue with germline testing of peripheral blood. An 18\u2010gene NGS panel was used to test tumor and germline DNA (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009106 patients). In 26 patients, 27 tumor Tier I or II variants were identified, with 16\/27 (59%) being germline pathogenic variants (PV) (13\n                    <jats:italic>BRCA1\/2<\/jats:italic>\n                    ; 3 other genes) and 11\/27 (41%) somatic variants (9\n                    <jats:italic>BRCA1\/2<\/jats:italic>\n                    ; 2 other). In 51\/106 patients, there were no tumor variants (excluding\n                    <jats:italic>TP53<\/jats:italic>\n                    ), of which one patient had a germline\n                    <jats:italic>BRCA1<\/jats:italic>\n                    copy number variant deletion in exon 12. Tumor\u2010first testing detected variant\u2010positive and variant\u2010negative germline cases in 105\/106 patients (99.1%). Among 50\n                    <jats:italic>BRCA<\/jats:italic>\n                    \u2010negative patients, 14\/50 (28%) were homologous recombination deficiency (HRD)\u2010positive. Therefore, we demonstrate that multigene NGS tumor\u2010testing is effective in identifying germline variants in EOC with a &lt;\u20091% false\u2010negative rate.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.70136","type":"journal-article","created":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T08:25:43Z","timestamp":1759652743000},"page":"838-849","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tumor and germline testing with next generation sequencing in epithelial ovarian cancer: a prospective paired comparison using an 18\u2010gene panel"],"prefix":"10.1002","volume":"20","author":[{"given":"Elisabeth","family":"Spenard","sequence":"first","affiliation":[{"name":"Division of Gynecologic Oncology University of Toronto  Canada"},{"name":"Division of Gynecologic Oncology Princess Margaret Cancer Centre, University Health Network  Canada"},{"name":"Institute of Health Policy Management and Evaluation, University of Toronto  Canada"},{"name":"Division of Gynecologic Oncology Laval University  Qu\u00e9bec City Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5499-240X","authenticated-orcid":false,"given":"Cristina","family":"Mitric","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology University of Toronto  Canada"},{"name":"Division of Gynecologic Oncology Princess Margaret Cancer Centre, University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Care","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Program, Division of Clinical Laboratory Genetics University Health Network  Canada"},{"name":"Department of Molecular Genetics University of Toronto  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4476-9722","authenticated-orcid":false,"given":"Tracy L.","family":"Stockley","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Program, Division of Clinical Laboratory Genetics University Health Network  Canada"},{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Bhalwani Familial Cancer Clinic Princess Margaret Cancer Centre, University Health Network  Canada"},{"name":"Division of Medical Oncology Department of Medicine, University of Toronto  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanna","family":"McCuaig","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics University of Toronto  Canada"},{"name":"Bhalwani Familial Cancer Clinic Princess Margaret Cancer Centre, University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaise","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ranich","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Program, Division of Clinical Laboratory Genetics University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Sheen","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Program, Division of Clinical Laboratory Genetics University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Ferguson","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology University of Toronto  Canada"},{"name":"Division of Gynecologic Oncology Princess Margaret Cancer Centre, University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liat","family":"Hogen","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology University of Toronto  Canada"},{"name":"Division of Gynecologic Oncology Princess Margaret Cancer Centre, University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taymaa","family":"May","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Dana Farber Cancer Institute, Harvard Medical School  Boston USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Q.","family":"Bernardini","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology University of Toronto  Canada"},{"name":"Division of Gynecologic Oncology Princess Margaret Cancer Centre, University Health Network  Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21456"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2024.0052"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.01.020"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2015.5495"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2016.90"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115052108"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.02960"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2023.12.010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1810858"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet\u20102018\u2010105472"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2023.0051"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2017.06.002"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jogc.2018.05.046"},{"key":"e_1_2_11_15_1","unstructured":"OntarioCC.Hereditary 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