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However, most studies on eccDNA have been conducted on tumor tissue or cancer cell lines, and limited research has been done on eccDNA in plasma. In this study, we investigated eccDNA in non\u2010small cell lung cancer (NSCLC) by sequencing plasma eccDNA from 32 epidermal growth factor receptor (\n                    <jats:italic>EGFR<\/jats:italic>\n                    )\u2010mutated NSCLC patients before and during treatment with osimertinib, as well as plasma eccDNA from five healthy individuals. Plasma eccDNA was identified in all samples but with significantly higher levels in cancer patients than healthy controls.\n                    <jats:italic>EGFR<\/jats:italic>\n                    \u2010overlapping eccDNA, eccDNA that contains part of or the whole\n                    <jats:italic>EGFR<\/jats:italic>\n                    gene, was detected in the majority of samples both at baseline and during treatment. High levels of\n                    <jats:italic>EGFR<\/jats:italic>\n                    \u2010overlapping eccDNA during osimertinib treatment were associated with significantly shorter progression\u2010free survival and overall survival. Plasma eccDNA represents a newly identified type of biomarker for monitoring treatment efficacy.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.70138","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T14:18:16Z","timestamp":1761833896000},"page":"1061-1073","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasma extrachromosomal circular\n                    <scp>DNA<\/scp>\n                    as a biomarker in\n                    <scp>EGFR<\/scp>\n                    \u2010targeted therapy of non\u2010small cell lung cancer"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9799-2434","authenticated-orcid":false,"given":"Simone","family":"Stensgaard","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Aarhus University Hospital  Denmark"},{"name":"Department of Clinical Medicine Aarhus University  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