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Modular CARs targeting multiple antigens have been proposed as an interesting solution to address these challenges by reducing the likelihood of tumor cells evading treatment through the loss of a single antigen. In this study, we present a new modular CAR platform, termed CARtein, which takes advantage of intein interactions to jointly target CD19 and CD20 antigens. We demonstrate that the CARtein system, which features a universal CAR signaling backbone that covalently binds to specific scFv\u2010intein recognition partners, generates fully active CARs. Functionality was validated using Raji cells and K562 cells expressing CD19 and\/or CD20, observing significant T\u00a0cell activation through NFAT and NF\u03baB promoter activity and CD69 upregulation. 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