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To advance\n                    <jats:italic>in vivo<\/jats:italic>\n                    diagnostics for OPSCC, Nanosecond infrared laser (NIRL) tissue sampling combined with shotgun lipidomic analysis reveals lipidome differences between OPSCC tissue and adjacent healthy tissue. In this study, ablations were performed on tonsil squamous cell carcinoma in 28 samples from 11 patients using an established chamber setup, and a subset of six samples from three patients with a custom\u2010made laser fiber\u2010coupled applicator, designed for handheld use. Welch's\n                    <jats:italic>t<\/jats:italic>\n                    \u2010test results (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.05, two\u2010fold change) revealed a similar OPSCC lipid profile in seven out of 11 patients. Potential tumor lipid markers were identified as consistently and significantly increased, despite the biological heterogeneity of the samples, underscoring their potential diagnostic value. Tissue ablation with fiber\u2010coupled applicator was successful and the lipidomic analysis was consistent with the chamber setup. While limited to off\u2010line analysis, our approach highlights the potential of fiber\u2010based laser sampling as a rapid and minimally invasive method to obtain tissue material for advanced molecular profiling in surgical and endoscopic settings.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.70188","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T07:56:02Z","timestamp":1766562962000},"page":"1513-1534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Infrared laser sampling of low volumes combined with shotgun lipidomics reveals lipid markers in palatine tonsil carcinoma"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8170-4346","authenticated-orcid":false,"given":"Leonard","family":"Kerkhoff","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology University Medical Center Hamburg\u2010Eppendorf  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