{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:55:27Z","timestamp":1789563327909,"version":"build-2803163510"},"reference-count":74,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2018\/31\/B\/NZ5\/01423"],"award-info":[{"award-number":["2018\/31\/B\/NZ5\/01423"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2023\/51\/B\/NZ4\/01016"],"award-info":[{"award-number":["2023\/51\/B\/NZ4\/01016"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>\n                    Receptor\u2010interacting protein kinase 4 (RIPK4) has been implicated in the progression of numerous tumours. In nonmelanoma skin cancer, RIPK4 plays a suppressor role, whereas in melanoma, it functions as an oncogene that modulates key signalling pathways involved in melanoma cell survival and expansion. Increased RIPK4 levels in metastatic melanoma biopsies prompted us to investigate the consequences of RIPK4 loss for the invasive and metastatic phenotype of melanoma cells. Using an integrated approach involving clinical samples,\n                    <jats:italic>in vivo<\/jats:italic>\n                    xenograft models, transcriptomic analysis and 3D functional assays, we show that RIPK4 deletion significantly reduces pulmonary metastasis formation. This reflects its role in late\u2010stage metastatic events, such as extravasation and colonization, particularly since this phenotype correlates with extensive transcriptional reprogramming of adhesion\u2010 and motility\u2010related genes in melanoma cells, as evidenced by next\u2010generation sequencing and functional validation in spheroid and collagen\u2010based models. Despite exhibiting features of a partial shift towards an amoeboid phenotype such as membrane blebbing and increased MLC2 phosphorylation, RIPK4 knockout cells display impaired motility and invasion. Re\u2010expression of RIPK4 restores mesenchymal morphology and migratory capacity. Together, our results establish RIPK4 as a critical regulator of melanoma invasion and metastasis. Nonetheless, they also demonstrate that the loss of RIPK4 function activates compensatory phenotypic shifts in melanoma cells that fail to fully rescue their invasive potential.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.70220","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T12:06:29Z","timestamp":1770638789000},"page":"1115-1139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>RIPK<\/scp>\n                    4 function interferes with melanoma cell adhesion and metastasis"],"prefix":"10.1002","volume":"20","author":[{"given":"Norbert","family":"Wronski","sequence":"first","affiliation":[{"name":"Department of Biophysics and Cancer Biology, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University  Krakow Poland"},{"name":"Doctoral School of Exact and Natural Sciences Jagiellonian University  Krakow 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Biological and Veterinary Sciences, Institute of Advanced Studies Nicolaus Copernicus University  Toru\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9548-1868","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Szczygie\u0142","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Cancer Biology, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University  Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3487-262X","authenticated-orcid":false,"given":"Agnieszka","family":"Harazin\u2010Lechowska","sequence":"additional","affiliation":[{"name":"Department of Tumour Pathology Maria Sklodowska\u2010Curie National Research Institute of Oncology, Krakow Branch  Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Czerbniak","sequence":"additional","affiliation":[{"name":"Department of 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