{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T09:08:24Z","timestamp":1781773704674,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["TRANSCAN\u2010199 Grant PROLIPSY"],"award-info":[{"award-number":["TRANSCAN\u2010199 Grant PROLIPSY"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"abstract":"<jats:p>\n                    Early detection of cancer is essential for effective treatment. However, current prostate cancer screening methods lack sufficient sensitivity and specificity, leading to overdiagnosis and unnecessary treatment. There is also an unmet need to distinguish clinically significant from insignificant prostate cancer. To identify complementary biomarkers for improved screening and diagnosis, we performed transcriptional profiling of cancer\u2010associated transcripts in circulating extracellular vesicles (EVs) isolated from peripheral blood of patients with suspected prostate cancer prior to biopsy and healthy donors. Expression data for 2549 mRNAs were obtained from 28 men. CAPN5 expression was significantly lower, whereas\n                    <jats:italic>BIRC2, CASP3, CD63, FMO5, IRF6, PFDN1, PRDX6, PSMD2, RIT1, S100A2, THBS1<\/jats:italic>\n                    , and\n                    <jats:italic>XRCC2<\/jats:italic>\n                    were significantly elevated in EVs from patients with significant prostate cancer (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200914) compared with cancer\u2010free individuals and patients with insignificant disease (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200914). Candidate biomarkers were subsequently evaluated by\n                    <jats:italic>in silico<\/jats:italic>\n                    validation using the The Cancer Genome Atlas (TCGA) prostate adenocarcinoma dataset and the GEO dataset\u00a0\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE70768\">GSE70768<\/jats:ext-link>\n                    containing benign and malignant prostate tissues. This analysis identified\n                    <jats:italic>CASP3<\/jats:italic>\n                    ,\n                    <jats:italic>XRCC2<\/jats:italic>\n                    , and\n                    <jats:italic>RIT1<\/jats:italic>\n                    transcripts in circulating EVs as promising biomarkers for the early detection of significant prostate cancer.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.70244","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T12:33:08Z","timestamp":1774528388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2451-9371","authenticated-orcid":false,"given":"Stefan","family":"Werner","sequence":"first","affiliation":[{"name":"Department of Tumor Biology University Medical Center Hamburg\u2010Eppendorf  Germany"},{"name":"The PROLIPSY consortium  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Tennstedt","sequence":"additional","affiliation":[{"name":"Martini\u2010Klinik Prostate Cancer Center, University Hospital Hamburg\u2010Eppendorf  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randi C.","family":"Pose","sequence":"additional","affiliation":[{"name":"Martini\u2010Klinik Prostate Cancer Center, University Hospital Hamburg\u2010Eppendorf  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Bioinformatics Core, University Medical Center Hamburg\u2010Eppendorf  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Besler","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology University Medical Center Hamburg\u2010Eppendorf  Germany"},{"name":"The PROLIPSY consortium  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svenja","family":"Schneegans","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology University Medical Center Hamburg\u2010Eppendorf  Germany"},{"name":"The PROLIPSY consortium  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malik","family":"Alawi","sequence":"additional","affiliation":[{"name":"Bioinformatics Core, University Medical Center Hamburg\u2010Eppendorf  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie C.","family":"Roesch","sequence":"additional","affiliation":[{"name":"Department of Urology University Hospital Schleswig\u2010Holstein, Campus L\u00fcbeck  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Peine","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine University Medical Center Hamburg\u2010Eppendorf  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree","family":"Bonci","sequence":"additional","affiliation":[{"name":"The PROLIPSY consortium  Hamburg Germany"},{"name":"Istituto Superiore di Sanit\u00e0  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9285-5417","authenticated-orcid":false,"given":"Joanna","family":"Budna\u2010Tukan","sequence":"additional","affiliation":[{"name":"The PROLIPSY consortium  Hamburg Germany"},{"name":"Department of Immunology Poznan University of Medical Sciences  Poznan Poland"},{"name":"Department of Histology and Embryology Poznan University of Medical Sciences  Poznan Poland"},{"name":"Department of Anatomy and Histology, Collegium Medicum University of Zielona Gora  Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7796-5914","authenticated-orcid":false,"given":"Evi","family":"Lianidou","sequence":"additional","affiliation":[{"name":"The PROLIPSY consortium  Hamburg Germany"},{"name":"Department of Analytical Chemistry University of Athens  Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6401-2903","authenticated-orcid":false,"given":"Catherine","family":"Alix\u2010Panabi\u00e8res","sequence":"additional","affiliation":[{"name":"The PROLIPSY consortium  Hamburg Germany"},{"name":"Laboratory of Rare Human Circulating Cells, University Medical Centre of Montpellier IURC  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derya","family":"Tilki","sequence":"additional","affiliation":[{"name":"Martini\u2010Klinik Prostate Cancer Center, University Hospital Hamburg\u2010Eppendorf  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5736-2772","authenticated-orcid":false,"given":"Klaus","family":"Pantel","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology University Medical Center Hamburg\u2010Eppendorf  Germany"},{"name":"The PROLIPSY consortium  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2019.08.005"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-022-00638-6"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/JU.0000000000001578"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2013.05.049"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-1311"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389-022-00431-5"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omto.2023.07.004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.505"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/pcan.2015.40"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.23901"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.24792"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2024.06.003"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2016.08.012"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.24536"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41391-021-00456-8"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.08.019"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2016.0097"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-021-01610-8"},{"key":"e_1_2_11_20_1","unstructured":"Antonio ChiesiNZ Davide Zocco.\u201cMethods and kits relating to the capture of CA\u2010IX positive exosomes. Patent number: 12404554.\u201d2025."},{"issue":"5","key":"e_1_2_11_21_1","article-title":"When less is more: specific capture and analysis of tumor exosomes in plasma increases the sensitivity of liquid biopsy for comprehensive detection of multiple androgen receptor phenotypes in advanced prostate cancer patients","volume":"8","author":"Foroni C","year":"2020","journal-title":"Biomedicine"},{"issue":"6","key":"e_1_2_11_22_1","first-page":"2133","article-title":"Feasibility of tumor\u2010derived exosome enrichment in the onco\u2010hematology leukemic model of chronic myeloid leukemia","volume":"44","author":"Bernardi S","year":"2019","journal-title":"International Journal of Molecular Medicine"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24076122"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2931"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0095"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.022"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.07.017"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04424-9"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.1075751"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81683-9"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.2021.1607"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1839-z"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks270"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-05-0400"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20200442"},{"issue":"9","key":"e_1_2_11_38_1","first-page":"10315","article-title":"Elevated expression of RIT1 correlates with poor prognosis in endometrial cancer","volume":"8","author":"Xu F","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12978"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610504104"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.70244","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1878-0261.70244","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.70244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:23:30Z","timestamp":1774563810000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.70244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,26]]},"references-count":39,"alternative-id":["10.1002\/1878-0261.70244"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.70244","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/1878-0261.70244\/v1\/decision1","asserted-by":"object"}]},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,26]]},"assertion":[{"value":"2026-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1878-0261.70244"}}