{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:15:26Z","timestamp":1792444526685,"version":"4.5.0"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","award":["MSCA4Ukraine project"],"award-info":[{"award-number":["MSCA4Ukraine project"]}],"id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["24\u201011793S"],"award-info":[{"award-number":["24\u201011793S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oncology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Osteosarcoma is the most common malignant bone tumor, primarily affecting adolescents and young adults. Patients with metastases have a low survival rate, making the identification of prognostic markers crucial. The adaptor protein CIN85 is involved in various signaling pathways that regulate cell differentiation, adhesion, and motility. Its overexpression is associated with poor prognosis in multiple cancers. However, the role of CIN85 in osteosarcoma progression has not yet been explored. This study shows that CIN85 expression is higher in osteosarcoma than in normal bone tissue and further increased in metastatic lesions relative to primary tumors. CIN85 overexpression increases cell migration and Matrigel invasion, whereas silencing CIN85 suppresses these behaviors. Functional annotation and enrichment analyses of the CIN85\u2010driven transcriptome suggest that CIN85 regulates migration, adhesion, and extracellular matrix organization in osteosarcoma. CIN85 affects\n                    <jats:italic>MMP2<\/jats:italic>\n                    and\n                    <jats:italic>COL3A1<\/jats:italic>\n                    gene expression and activates Akt\/mTOR signaling. Knockdown of\u00a0\n                    <jats:italic>MMP2<\/jats:italic>\n                    and\n                    <jats:italic>COL3A1<\/jats:italic>\n                    or pharmacological inhibion of Akt\/mTOR signaling abrogates CIN85\u2010induced motility. This study demonstrates that elevated CIN85 expression contributes to osteosarcoma migration and metastasis, highlighting its potential as a therapeutic target.\n                  <\/jats:p>","DOI":"10.1002\/1878-0261.70245","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:49:27Z","timestamp":1776487767000},"page":"2085-2101","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Adaptor protein\n                    <scp>CIN85<\/scp>\n                    potentiates the motility of osteosarcoma cells via the Akt\/\n                    <scp>mTOR<\/scp>\n                    and\n                    <scp>MMP2<\/scp>\n                    \u2010\n                    <scp>COL3A1<\/scp>\n                    axis"],"prefix":"10.1002","volume":"20","author":[{"given":"Iryna","family":"Horak","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Experimental Biology Masaryk University  Brno Czech Republic"},{"name":"Department of Cell Signaling Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iva Staniczkov\u00e1","family":"Zambo","sequence":"additional","affiliation":[{"name":"International Clinical Research Center, St. Anne's University Hospital  Brno Czech Republic"},{"name":"1st Department of Pathology, St. Anne's University Hospital and Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u011bj","family":"P\u0159ikryl","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Experimental Biology Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"M\u00fadry","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, University Hospital Brno and Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danica","family":"Zapletalov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, University Hospital Brno and Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarmila","family":"Navr\u00e1tilov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Experimental Biology Masaryk University  Brno Czech Republic"},{"name":"International Clinical Research Center, St. Anne's University Hospital  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"Navr\u00e1til","sequence":"additional","affiliation":[{"name":"Department of Pathological Physiology, Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"\u0160marda","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Experimental Biology Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liudmyla","family":"Drobot","sequence":"additional","affiliation":[{"name":"Department of Cell Signaling Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Bene\u0161","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Experimental Biology Masaryk University  Brno Czech Republic"},{"name":"International Clinical Research Center, St. Anne's University Hospital  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4897-0404","authenticated-orcid":false,"given":"Lucia","family":"Knopfov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Experimental Biology Masaryk University  Brno Czech Republic"},{"name":"International Clinical Research Center, St. Anne's University Hospital  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971\u20105851.203513"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2024.100521"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2024.189171"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2018.11.027"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2020.151654"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1586\/era.09.69"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2025.1699287"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/or.2025.1630239"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100854.2010.01061.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1265"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs228"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201411025"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404\u2010010\u20101693\u20109"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11650"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers7010342"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.583984"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20105956\u20107\u201021"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6286"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052622"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14265"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100503\u20106"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/937506"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585\u2010021\u201010145\u20104"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b02462"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmeasuresciau.5c00121"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw377"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.17912\/MICROPUB.BIOLOGY.000811"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj109"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3233\/cbm-190562"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0241140"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-024-09840-1"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041388"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43465\u2010020\u201000098\u20103"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14227952"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.7124\/bc.000981"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH09\u201008\u20100524"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201018\u20103852"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00133"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2023.100513"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409839200"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00522"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/416183a"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205535200"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.120"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.2337\/db16\u20100081"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S273466"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.10.032"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.15.4015"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.08.075"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.004333"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601534"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010023\u201000722\u20106"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010024\u201000778\u2010y"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/cnr2.1946"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.911972"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202510"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgac020"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010022\u201009874\u2010w"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14202"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2021.108895"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108155"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22042208"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020534"}],"container-title":["Molecular Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.70245","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1878-0261.70245","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1878-0261.70245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T19:29:44Z","timestamp":1785958184000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/1878-0261.70245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,17]]},"references-count":63,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/1878-0261.70245"],"URL":"https:\/\/doi.org\/10.1002\/1878-0261.70245","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/1878-0261.70245\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/1878-0261.70245\/v1\/review1","asserted-by":"object"}]},"ISSN":["1574-7891","1878-0261"],"issn-type":[{"value":"1574-7891","type":"print"},{"value":"1878-0261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,17]]},"assertion":[{"value":"2025-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}