{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:13:51Z","timestamp":1786976031619,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001922","name":"Efficacy and Mechanism Evaluation Programme","doi-asserted-by":"publisher","award":["128103"],"award-info":[{"award-number":["128103"]}],"id":[{"id":"10.13039\/501100001922","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The GUSTO clinical trial (Gene expression subtypes of Urothelial carcinoma: Stratified Treatment and Oncological outcomes) uses molecular subtypes to guide neoadjuvant therapies in participants with muscle\u2010invasive bladder cancer (MIBC). Before commencing the GUSTO trial, we needed to determine the reliability of a commercial subtyping platform (Decipher Bladder; Veracyte) when performed in an external trial laboratory as this has not been done previously. Here, we report our pre\u2010trial verification of the TCGA molecular subtyping model using gene expression profiling. Formalin\u2010fixed paraffin\u2010embedded tissue blocks of MIBC were used for gene expression subtyping by gene expression microarrays. Intra\u2010 and inter\u2010laboratory technical reproducibilities, together with quality control of laboratory and bioinformatics processes, were assessed. Eighteen samples underwent analysis. RNA of sufficient quality and quantity was successfully extracted from all samples. All subtypes were represented in the cohort. Each sample was subtyped twice in our laboratory and once in a separate reference laboratory. No clinically significant discordance in subtype occurred between intra\u2010 or inter\u2010laboratory replicates. Examination of sample histopathology showed variability of morphological appearances within and between subtypes. Overall, these results show that molecular subtyping by gene expression profiling is reproducible, robust and suitable for use in the GUSTO clinical trial.<\/jats:p>","DOI":"10.1002\/2056-4538.12363","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:33:02Z","timestamp":1706833982000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Verification of molecular subtyping of bladder cancer in the\n                    <scp>GUSTO<\/scp>\n                    clinical trial"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3969-7637","authenticated-orcid":false,"given":"Jon","family":"Griffin","sequence":"first","affiliation":[{"name":"Department of Histopathology Sheffield Teaching Hospitals NHS Foundation Trust  Sheffield UK"},{"name":"Academic Urology Unit, Division of Clinical Medicine University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Down","sequence":"additional","affiliation":[{"name":"Academic Urology Unit, Division of Clinical Medicine University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis A","family":"Quayle","sequence":"additional","affiliation":[{"name":"Academic Urology Unit, Division of Clinical Medicine University of Sheffield  Sheffield UK"},{"name":"Department of Computing Sheffield Hallam University  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R","family":"Heath","sequence":"additional","affiliation":[{"name":"Sheffield Institute for Translational Neuroscience University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewan A","family":"Gibb","sequence":"additional","affiliation":[{"name":"Veracyte Inc  South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elai","family":"Davicioni","sequence":"additional","affiliation":[{"name":"Veracyte Inc  South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Veracyte Inc  South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Veracyte Inc  South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayne","family":"Swain","sequence":"additional","affiliation":[{"name":"Clinical Trials Research Unit University of Leeds  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Wang","sequence":"additional","affiliation":[{"name":"Sheffield Institute for Translational Neuroscience University of Sheffield  Sheffield UK"},{"name":"National Heart and Lung Institute Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed","family":"Hussain","sequence":"additional","affiliation":[{"name":"Academic Oncology Unit, Division of Clinical Medicine University of Sheffield  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Crabb","sequence":"additional","affiliation":[{"name":"Southampton Experimental Cancer Medicine Centre University of Southampton  Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James WF","family":"Catto","sequence":"additional","affiliation":[{"name":"Academic Urology Unit, Division of Clinical Medicine University of Sheffield  Sheffield UK"},{"name":"Department of Urology Sheffield Teaching Hospitals NHS Foundation Trust  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the GUSTO Trial Management Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2023.03.029"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15970"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.7393"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-020-00733-z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2005.04.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2019.09.006"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2017.03.030"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.007"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.01.009"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/JU.0000000000002261"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-18-3558"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/9349132"},{"key":"e_1_2_8_15_1","unstructured":"MIBCConsensus classification. [Accessed 18 November 2022]. Available from:https:\/\/github.com\/cit-bioinfo\/consensusMIBC"},{"key":"e_1_2_8_16_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2013"},{"key":"e_1_2_8_17_1","first-page":"64","article-title":"Current concept for tertiary lymphoid structures in urothelial carcinoma of the bladder: a literature review and our experience","volume":"9","author":"Gulinac M","year":"2020","journal-title":"Am J Clin Exp Immunol"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-021-00232-6"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp-2023-209017"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2021.153611"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0215138"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12993"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2019-206236"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204808"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3791\/62394"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-019-0643-z"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-449"},{"key":"e_1_2_8_28_1","unstructured":"Qiagen.Gene expression and function studies. 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