{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:11:42Z","timestamp":1785859902252,"version":"3.56.0"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019223","name":"Ministry of Health \u2013 Kingdom of Saudi Arabia","doi-asserted-by":"publisher","award":["FTN 00064190"],"award-info":[{"award-number":["FTN 00064190"]}],"id":[{"id":"10.13039\/100019223","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Several prognostic markers, including tumor\u2010infiltrating lymphocytes, which have been recently identified in penile squamous cell carcinoma (pSCC), have focused mostly on T cells. The prognostic role of B cells and tertiary lymphoid structures (TLSs) has not yet been sufficiently described. We examined whole tissue sections histopathologically for TLSs and immunohistochemically for CD20 and CD138. The B\u2010cell immunoscore (B\u2010IS) divided the cohort into five categories based on the expression of these two B\u2010cell\/plasma cell markers (CD20 and CD138, respectively). Patients with fewer TLSs had worse overall survival (OS) [hazard ratio (HR)\u2009=\u20092.17; 95% CI: 0.94\u20135;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.069]. A significant association was identified between a high TLS diameter and the presence of a lymphocytic infiltrate [odds ratio (OR)\u2009=\u20092.2442; 95% CI: 1.1022\u20134.55;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0208]. Patients with low B\u2010IS (HR\u2009=\u20091.89, 95% CI: 1.18\u20133.03,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.008), a low number of CD20\n                    <jats:sup>+<\/jats:sup>\n                    cells in the tumor center (HR\u2009=\u20091.67, 95% CI: 1.04\u20132.7,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.035), and a low number of CD20\n                    <jats:sup>+<\/jats:sup>\n                    cells at the tumor invasion front (HR\u2009=\u20091.69, 95% CI: 1.06\u20132.78;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.028) had significantly worse OS. High B\u2010ISs were strongly associated with a mutated p53 profile detected by immunohistochemistry (OR\u2009=\u20094.76, 95% CI: 1.32\u201325,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.011), low T\u2010cell immunoscores (OR\u2009=\u20090.49; 95% CI: 0.23\u20131.03;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.051), and brisk lymphocytic infiltration (OR\u2009=\u20092.0417, 95% CI: 1.01\u20134.76;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.037). High CD20\n                    <jats:sup>+<\/jats:sup>\n                    cell counts at the invasion front were associated with histological grade 3 disease (OR\u2009=\u20092.44, 95% CI 1.15\u20135.26,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.015). An association was also observed between low B\u2010IS and mutations in\n                    <jats:italic>KMT2D<\/jats:italic>\n                    (OR 0.31, 95% CI: 0.07\u20131.21,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.057) and\n                    <jats:italic>EGFR<\/jats:italic>\n                    (OR\u2009=\u2009\u221e, 95% CI: 0.86\u2013\u221e,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.053). In conclusion, high numbers of tumor\u2010infiltrating B cells within TLSs represent a favorable prognostic marker in pSCC. These findings emphasize the need to identify novel microscopic prognostic markers during pathological assessment to guide early and appropriate therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1002\/2056-4538.70059","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T11:49:06Z","timestamp":1763120946000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["B lymphocytes and tertiary lymphoid structures have a prognostic impact on penile squamous cell carcinoma"],"prefix":"10.1002","volume":"11","author":[{"given":"Nicolette","family":"Zavillov\u00e1","sequence":"first","affiliation":[{"name":"Department of Urology, 3rd Faculty of Medicine of Charles University Thomayer University Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4668-5837","authenticated-orcid":false,"given":"Petr","family":"Waldauf","sequence":"additional","affiliation":[{"name":"Department of Anesthesia and Intensive Care Medicine, 3rd Faculty of Medicine, Charles University University Hospital Kralovske Vinohrady  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5869-3618","authenticated-orcid":false,"given":"Michaela","family":"Kendall B\u00e1rt\u016f","sequence":"additional","affiliation":[{"name":"Department of Pathology, 1st Faculty of Medicine, Charles University General University Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"\u010capka","sequence":"additional","affiliation":[{"name":"Department of Urology, 3rd Faculty of Medicine, Charles University University Hospital Kralovske Vinohrady  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3563-2484","authenticated-orcid":false,"given":"Jan","family":"Hojn\u00fd","sequence":"additional","affiliation":[{"name":"Department of Pathology, 1st Faculty of Medicine, Charles University General University Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Prouzov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathology, 1st Faculty of Medicine, Charles University General University Hospital  Prague Czech Republic"},{"name":"Department of Pathology, 3rd Faculty of Medicine, Charles University University Hospital Kralovske Vinohrady  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6152-6343","authenticated-orcid":false,"given":"Radoslav","family":"Mat\u011bj","sequence":"additional","affiliation":[{"name":"Department of Pathology, 1st Faculty of Medicine, Charles University General University Hospital  Prague Czech Republic"},{"name":"Department of Pathology, 3rd Faculty of Medicine, Charles University University Hospital Kralovske Vinohrady  Prague Czech Republic"},{"name":"Department of Pathology and Molecular Medicine, 3rd Faculty of Medicine, Charles University Thomayer University Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0906-269X","authenticated-orcid":false,"given":"Roman","family":"Zachoval","sequence":"additional","affiliation":[{"name":"Department of Urology, 3rd Faculty of Medicine of Charles University Thomayer University Hospital  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5649-4586","authenticated-orcid":false,"given":"Jan","family":"Hrudka","sequence":"additional","affiliation":[{"name":"Department of Pathology, 3rd Faculty of 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