{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:36:20Z","timestamp":1780090580408,"version":"3.54.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This prospective single\u2010center study aimed to investigate whether preserving cell\u2010free ribonucleic acid (cfRNA) in specimens' supernatants (SS) obtained from percutaneous core\u2010needle biopsy (CNB) of non\u2010small cell lung cancer (NSCLC) can improve the performance of genomic testing. Forty\u2010three NSCLC patients who underwent image\u2010guided CNB were enrolled. The SS from each CNB specimen was divided into two parts: one was mixed 1:1 with a cfRNA\u2010protective solution, and the other was left unhandled. For quantitative analysis, 30 patients (regardless of mutation status) were evaluated by comparing cfRNA yield between cfRNA\u2010protected and \u2010unprotected SS. For qualitative analysis, 15 patients with fusion gene alterations were assessed by comparing genotyping results from cfRNA\u2010protected SS to those from paired CNB specimens. Pneumothorax was the most frequent adverse event in CNB procedures, occurring in 20.9% of cases (9\/43). No one experienced severe adverse events. In the quantitative analysis, 90.0% (27\/30) of cfRNA\u2010protected SS yielded adequate cfRNA, significantly higher than 53.3% (16\/30) in cfRNA\u2010unprotected SS (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). In the qualitative analysis, despite ineffective cfRNA preservation in three cases, DNA\u2010level mutations were still detected in both the CNB specimens and cfRNA\u2010protected SS. The overall concordance of genotyping results between cfRNA\u2010protected SS and paired CNB specimens was 100%, correctly identifying all\n                    <jats:italic>ALK<\/jats:italic>\n                    fusions,\n                    <jats:italic>ROS1<\/jats:italic>\n                    rearrangements, and\n                    <jats:italic>MET\u201014<\/jats:italic>\n                    skipping alterations. These findings highlight that preserving cfRNA in CNB\u2010SS from NSCLC can improve the performance of genomic testing, particularly for RNA\u2010based assays, without compromising the accuracy of DNA\u2010based assays.\n                  <\/jats:p>","DOI":"10.1002\/2056-4538.70081","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:23:11Z","timestamp":1773004991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preservation of cell\u2010free\n                    <scp>RNA<\/scp>\n                    in percutaneous core\u2010needle biopsy specimens' supernatants from non\u2010small cell lung cancer improves genomic testing performance"],"prefix":"10.1002","volume":"12","author":[{"given":"Sheng","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Minimally Invasive Tumor Therapies Center, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Dan","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"},{"name":"Department of Pathology, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Xin","family":"Bie","sequence":"additional","affiliation":[{"name":"Department of Minimally Invasive Tumor Therapies Center, Beijing Hospital, National Center of Gerontology Institute of Geriatric Medicine, Chinese Academy of Medical Sciences  Beijing 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