{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:38:12Z","timestamp":1792741092283,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology CR"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Epidermal growth factor receptor (EGFR), cellular mesenchymal\u2010epithelial transition factor (c\u2010MET), and B7 homolog 3 (B7H3) are important targets for antibody\u2010based drug development in colorectal cancer (CRC). To overcome the limitations of single\u2010target antibody therapies \u2013 such as limited efficacy, widespread resistance, narrow patient populations, and treatment\u2010related toxicities \u2013 various bispecific antibodies or antibody\u2013drug conjugate (ADCs) targeting two targets are being extensively validated in clinical settings. A deeper understanding of their expression profiles and co\u2010expression patterns of actionable therapeutic targets may guide more effective treatment strategies. We evaluated the immunohistochemical expression of B7H3, c\u2010MET, and EGFR in a cohort of 193 CRC patients. Among which, B7H3 exhibited the highest positivity rate (80.83%) in CRC tissues, followed by c\u2010MET (77.20%) and EGFR (47.15%). The positive rates of B7H3 showed no significant differences across patient gender, age, TNM stage, differentiation grade, or tumor site. In contrast, the c\u2010MET positivity rate was elevated in patients with stage I\u2013II disease, and the expression of EGFR was significantly higher in female patients than in male patients. Concurrent assessment of the three targets in metastatic lesions revealed that B7H3 expression levels were comparable between primary and metastatic tumors, whereas c\u2010MET expression was significantly higher in primary lesions. Co\u2010expression analysis indicated that the group with the highest patient coverage was the B7H3\/c\u2010MET dual\u2010positive group, followed by the B7H3\/EGFR, and c\u2010MET\/EGFR combinations, and the proportion of patients with at least one positive target exceeded 80%. Finally, mRNA profile from TIMER database reveals that\n                    <jats:italic>CD276<\/jats:italic>\n                    and\n                    <jats:italic>MET<\/jats:italic>\n                    expression in CRC tumor tissues were higher than those in the majority of normal tissues. Therefore, utilizing B7H3\/c\u2010MET combination therapies that integrate intracellular signaling inhibition with immune checkpoint modulation could potentially extend therapeutic benefits to a wider range of CRC patients.\n                  <\/jats:p>","DOI":"10.1002\/2056-4538.70118","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:45:43Z","timestamp":1790743543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Profiling\n                    <scp>EGFR<\/scp>\n                    , c\u2010\n                    <scp>MET<\/scp>\n                    , and\n                    <scp>B7H3<\/scp>\n                    co\u2010expression to guide next\u2010generation dual\u2010drug conjugate strategies in colorectal cancer"],"prefix":"10.1002","volume":"12","author":[{"given":"Yanan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pathology, Hubei Cancer 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