{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:28:38Z","timestamp":1768969718534,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,10,20]],"date-time":"2016-10-20T00:00:00Z","timestamp":1476921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360309"],"award-info":[{"award-number":["81360309"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572738"],"award-info":[{"award-number":["81572738"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270898"],"award-info":[{"award-number":["81270898"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2014GXNSFAA118194"],"award-info":[{"award-number":["2014GXNSFAA118194"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2015GXNSFEA139003"],"award-info":[{"award-number":["2015GXNSFEA139003"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20151577"],"award-info":[{"award-number":["BK20151577"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Oversupply of free fatty acids such as palmitic acid (<jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>) from the portal vein may cause liver insulin resistance. Production of reactive oxygen species plays a pivotal role in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\u2010induced insulin resistance in H4<jats:styled-content style=\"fixed-case\">IIEC<\/jats:styled-content>3 hepatocytes. Recently, we found that exosomes secreted from <jats:styled-content style=\"fixed-case\">INS<\/jats:styled-content>\u20101 cells that were transfected with neutral ceramidase (<jats:styled-content style=\"fixed-case\">NCD<\/jats:styled-content>ase) plasmids had raised <jats:styled-content style=\"fixed-case\">NCD<\/jats:styled-content>ase activity; these <jats:styled-content style=\"fixed-case\">NCD<\/jats:styled-content>ase\u2010enriched exosomes could inhibit <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\u2010induced <jats:styled-content style=\"fixed-case\">INS<\/jats:styled-content>\u20101 cell apoptosis. Here, we showed that <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content> reduced insulin\u2010stimulated tyrosine phosphorylation of insulin receptor substrate 2 and decreased insulin\u2010stimulated uptake of the fluorescent glucose analog 2\u2010<jats:styled-content style=\"fixed-case\">NBDG<\/jats:styled-content>, confirming that insulin resistance occurred in <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\u2010treated H4<jats:styled-content style=\"fixed-case\">IIEC<\/jats:styled-content>3 cells. Moreover, <jats:styled-content style=\"fixed-case\">NCD<\/jats:styled-content>ase\u2010enriched exosomes from <jats:styled-content style=\"fixed-case\">INS<\/jats:styled-content>\u20101 cells rescued <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\u2010induced H4<jats:styled-content style=\"fixed-case\">IIEC<\/jats:styled-content>3 insulin resistance and blocked <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\u2010induced reactive oxygen species production in which ceramide was involved.<\/jats:p>","DOI":"10.1002\/2211-5463.12125","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T07:37:00Z","timestamp":1473147420000},"page":"1078-1084","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Neutral ceramidase\u2010enriched exosomes prevent palmitic acid\u2010induced insulin resistance in H4<scp>IIEC<\/scp>3 hepatocytes"],"prefix":"10.1002","volume":"6","author":[{"given":"Qun","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Endocrinology the Second Affiliated Hospital of Nanjing Medical University Jiangsu China"},{"name":"China\u2010USA Lipids in Health and Disease Research Center Guilin Medical University Guangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Hepatobiliary and Pancreatic Surgery Affiliated Hospital of Guilin Medical University Guangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfei","family":"Jin","sequence":"additional","affiliation":[{"name":"China\u2010USA Lipids in Health and Disease Research Center Guilin Medical University Guangxi China"},{"name":"Laboratory of Hepatobiliary and Pancreatic Surgery Affiliated Hospital of Guilin Medical University Guangxi China"},{"name":"Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair Guilin Medical University Guangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/414799a"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M901488200"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.1.3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21874"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01537.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v3.24641"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12826"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002522200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2008.00781.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20144238"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00251.2013"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-2187-8-12"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116858"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.107821"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2014.04.005"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102153200"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.04.009"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.121164498"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44844-7"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000035650.05921.50"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh0801.089604"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.6.4228"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-12-98"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.3.591"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801597200"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F2211-5463.12125","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.12125","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T15:16:38Z","timestamp":1696605398000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,20]]},"references-count":25,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/2211-5463.12125"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12125","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,20]]},"assertion":[{"value":"2016-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-08-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-10-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}