{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:39:26Z","timestamp":1791315566343,"version":"4.3.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T00:00:00Z","timestamp":1482192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["14XJ10010"],"award-info":[{"award-number":["14XJ10010"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Angiogenesis mediated by vascular endothelial growth factor (<jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>) is known to play an important role in regulating cartilage remodelling and endochondral ossification. However, the details of how <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> signalling mechanisms affect condyle remodelling in response to alterations in functional loading remains unclear. To explore this, eighty 16\u2010day\u2010old male <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content> rats were divided into two equal groups which were fed either a soft\/powdery diet or a hard diet for 4 weeks; the stiffness of the diet results in alteration of mastication force and hence temporomandibular joint (TMJ) development. We performed a proteomic analysis of rat condylar cartilage using isobaric tags for relative and absolute quantification (<jats:styled-content style=\"fixed-case\">iTRAQ<\/jats:styled-content>) labelling, followed by 2D nano\u2010high performance liquid chromatography and MALDI\u2010TOF\/time\u2010of\u2010flight technology. After protein identification, we used biological information analysis to identify the differentially expressed proteins associated with the <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> signalling pathway. Among the identified differentially expressed proteins, we found <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> signalling mainly <jats:italic>via<\/jats:italic> the p44\/42 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> and p38 mitogen\u2010activated protein kinase (<jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>) pathways in condylar cartilage, including <jats:styled-content style=\"fixed-case\">VEGFD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> VGFR<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">KPCB<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> KPCT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> KPCZ<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ARAF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> RASN<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PLCG<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">PLCG<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">JUN<\/jats:styled-content> and M3K12. Furthermore, four representative protein candidates, <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>, p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> and p44\/42 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>\/phospho\u2010p44\/42 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>, were confirmed by immunohistochemical staining and western blot. Our data suggest that <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> might play an important role in TMJ development and remodelling in response to alterations in functional loading through the p44\/42 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> and p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> signalling pathway. This study provides new clues to the understanding of the signalling mechanism responsible for <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> production in response to different masticatory functions at the protein level.<\/jats:p>","DOI":"10.1002\/2211-5463.12155","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T15:50:13Z","timestamp":1478533813000},"page":"44-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of the vascular endothelial growth factor signalling pathway by quantitative proteomic analysis of rat condylar cartilage"],"prefix":"10.1002","volume":"7","author":[{"given":"Liting","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Stomatology Ruijin Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine China"},{"name":"Department of Prosthodontics Shanghai Key Laboratory of Stomatology 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