{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:37:59Z","timestamp":1785307079049,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T00:00:00Z","timestamp":1500854400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T00:00:00Z","timestamp":1500854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19AI113170"],"award-info":[{"award-number":["U19AI113170"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007188","name":"Uniformed Services University of the Health Sciences","doi-asserted-by":"publisher","award":["R0713018"],"award-info":[{"award-number":["R0713018"]}],"id":[{"id":"10.13039\/100007188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p>Effective vaccines are urgently needed to combat gonorrhea, a common sexually transmitted bacterial infection, for which treatment options are diminishing due to rapid emergence of antibiotic resistance. We have used a rational approach to the development of gonorrhea vaccines, and genetically engineered nanoparticles to present antigenic peptides of <jats:italic>Neisseria\u00a0gonorrhoeae<\/jats:italic>, the causative agent of gonorrhea. We hypothesized that the ferritin nanocage could be used as a platform to display an ordered array of <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> antigenic peptides on its surface. MtrE, the outer membrane channel of the highly conserved gonococcal MtrCDE active efflux pump, is an attractive vaccine target due to its importance in protecting <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> from host innate effectors and antibiotic resistance. Using computational approaches, we designed constructs that expressed chimeric proteins of the <jats:italic>Helicobacter\u00a0pylori<\/jats:italic> ferritin and antigenic peptides that correspond to the two surface\u2010exposed loops of <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> MtrE. The peptides were inserted at the N terminus or in a surface\u2010exposed ferritin loop between helices \u03b1A and \u03b1B. Crystal structures of the chimeric proteins revealed that the proteins assembled correctly into a 24\u2010mer nanocage structure. Although the inserted <jats:italic>N.\u00a0gonorrhoeae<\/jats:italic> peptides were disordered, it was clear that they were displayed on the nanocage surface, but with multiple conformations. Our results confirmed that the ferritin nanoparticle is a robust platform to present antigenic peptides and therefore an ideal system for rational design of immunogens.<\/jats:p>","DOI":"10.1002\/2211-5463.12267","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T16:12:41Z","timestamp":1499098361000},"page":"1196-1207","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Structure\u2010based design of ferritin nanoparticle immunogens displaying antigenic loops of <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1002","volume":"7","author":[{"given":"Liqin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Uniformed Services University of the Health Sciences Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Uniformed Services University of the Health Sciences Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Le Van","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Uniformed Services University of the Health Sciences Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann E.","family":"Jerse","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Uniformed Services University of the Health Sciences Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuishu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Uniformed Services University of the Health Sciences Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,24]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"WHO(2012)Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. 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