{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:05:41Z","timestamp":1777442741405,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T00:00:00Z","timestamp":1503964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1RO1GM105898"],"award-info":[{"award-number":["1RO1GM105898"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>\n                    t\u2010Darpp (truncated isoform of dopamine\u2010 and\n                    <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\n                    \u2010regulated phosphoprotein) is a protein encoded by the\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">PPP<\/jats:styled-content>\n                      1R1B\n                    <\/jats:italic>\n                    gene and is expressed in breast, colon, esophageal, gastric, and prostate cancers, as well as in normal adult brain striatal cells. Overexpression of t\u2010Darpp in cultured cells leads to increased protein kinase A activity and increased phosphorylation of\n                    <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n                    (protein kinase B). In\n                    <jats:styled-content style=\"fixed-case\">HER<\/jats:styled-content>\n                    2+ breast cancer cells, t\u2010Darpp confers resistance to the chemotherapeutic agent trastuzumab. To shed light on t\u2010Darpp function, we studied its secondary structure, oligomerization status, metal\u2010binding properties, and phosphorylation by cyclin\u2010dependent kinases 1 and 5. t\u2010Darpp exhibits 12% alpha helix, 29% beta strand, 24% beta turn, and 35% random coil structures. It binds calcium, but not other metals commonly found in biological systems. The T39 site, critical for t\u2010Darpp activation of the\n                    <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n                    signaling pathway, is a substrate for phosphorylation by cyclin\u2010dependent kinase 1 and cyclin\u2010dependent kinase 5. Gel filtration chromatography, sedimentation equilibrium analysis, blue native gel electrophoresis, and glutaraldehyde\u2010mediated cross\u2010linking experiments demonstrate that the majority of t\u2010Darpp exists as a monomer, but forms low levels (&lt;\u00a03%) of hetero\u2010oligomers with its longer isoform Darpp\u201032. t\u2010Darpp has a large Stokes radius of 4.4\u00a0nm relative to its mass of 19\u00a0kDa, indicating that it has an elongated structure.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.12269","type":"journal-article","created":{"date-parts":[[2017,7,22]],"date-time":"2017-07-22T03:52:45Z","timestamp":1500695565000},"page":"1328-1337","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["t\u2010Darpp is an elongated monomer that binds calcium and is phosphorylated by cyclin\u2010dependent kinases 1 and 5"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2017-9733","authenticated-orcid":false,"given":"Jamil","family":"Momand","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrycja","family":"Magdziarz","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"You","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dianlu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University Los Angeles CA 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L.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Biochemistry Instrumentation University of California Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estuardo","family":"Monterroso","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan E.","family":"Kane","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology Beckman Research Institute at City of Hope Duarte CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feimeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry California State University Los Angeles CA 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