{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:22:25Z","timestamp":1790680945605,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T00:00:00Z","timestamp":1505952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472160"],"award-info":[{"award-number":["81472160"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:p>Ischemic stroke is a leading cause of death and disability worldwide, and autophagy may be involved in the pathological process of cerebral ischemia\/reperfusion injury. Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is an endogenous gasotransmitter with protective effects against multiple diseases. Here, we tested the effect of H<jats:sub>2<\/jats:sub>S on cerebral ischemia\/reperfusion injury in rats. Sodium hydrosulfide (Na<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>), an H<jats:sub>2<\/jats:sub>S donor, improved neurological function and reduced the size of the infarcts induced by transient middle cerebral artery occlusion (<jats:styled-content style=\"fixed-case\">MCAO<\/jats:styled-content>) followed by reperfusion in rats. Na<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> treatment reduced the lactate dehydrogenase (<jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content>) activity in the serum (a marker of cellular membrane integrity) and the expression of cleaved caspase\u20103 (a marker for apoptosis) in the brains of <jats:styled-content style=\"fixed-case\">MCAO<\/jats:styled-content> rats. We also found that autophagy was overactivated in the brains of <jats:styled-content style=\"fixed-case\">MCAO<\/jats:styled-content> rats, as indicated by an increased ratio of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3 <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> to I, decreased expression of p62, and transmission electron microscope detection. Na<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> treatment significantly inhibited the autophagic activity in the brains of <jats:styled-content style=\"fixed-case\">MCAO<\/jats:styled-content> rats. Furthermore, <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>12 cells were subjected to oxygen\u2013glucose deprivation\/reoxygenation (<jats:styled-content style=\"fixed-case\">OGD<\/jats:styled-content>\/R) to mimic <jats:styled-content style=\"fixed-case\">MCAO <\/jats:styled-content><jats:italic>in vitro<\/jats:italic>. We found that Na<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> treatment reduced cellular injury and suppressed overactivated autophagy induced by <jats:styled-content style=\"fixed-case\">OGD<\/jats:styled-content>\/R in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>12 cells. An autophagy stimulator (rapamycin) eliminated the protective effect of Na<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> against <jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content> release and caspase\u20103 activity induced by <jats:styled-content style=\"fixed-case\">OGD<\/jats:styled-content>\/R in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>12 cells. An autophagy inhibitor (3\u2010methyladenine, 3\u2010<jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>) also reduced the cellular injury induced by <jats:styled-content style=\"fixed-case\">OGD<\/jats:styled-content>\/R in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>12 cells. In conclusion, the results indicate that overactivated autophagy accelerates cellular injury after <jats:styled-content style=\"fixed-case\">MCAO<\/jats:styled-content> in rats and that exogenous H<jats:sub>2<\/jats:sub>S attenuates cerebral ischemia\/reperfusion injury via suppressing overactivated autophagy in rats.<\/jats:p>","DOI":"10.1002\/2211-5463.12301","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T16:16:40Z","timestamp":1502986600000},"page":"1686-1695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Sodium hydrosulfide attenuates cerebral ischemia\/reperfusion injury by suppressing overactivated autophagy in rats"],"prefix":"10.1002","volume":"7","author":[{"given":"Wen\u2010Wu","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Physiology Xiangya School of Medicine Central South University Changsha China"},{"name":"Department of Neurosurgery the First Affiliated Hospital of University of South China Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai\u2010Sheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Physiology Xiangya School of Medicine Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Physiology Xiangya School of Medicine Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lv\u2010Hong","family":"Deng","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of University of South China Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Xiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology Xiangya School of Medicine Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35885"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000200455"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-016-0409-5"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2403-y"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17030332"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8206"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2016.04.005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2016.08.026"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-014-1396-x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.03.019"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.06.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.06.200"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36125"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9049782"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2013.09.003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14080"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.12.013"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.003531"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2016-0100"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12825"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2012.11.003"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.27.9.1616"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/147323001204000314"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-43"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0370-y"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12005"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-013-0299-5"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules191015786"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2015.12.007"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.115.002125"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000430317"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfr560"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F2211-5463.12301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:26:31Z","timestamp":1696033591000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,21]]},"references-count":32,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/2211-5463.12301"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12301","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,21]]},"assertion":[{"value":"2017-04-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}