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More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we show that <jats:styled-content style=\"fixed-case\">FAD<\/jats:styled-content> treatment, using DMSO as a solvent, enabled an increase in the amount of intracellular <jats:styled-content style=\"fixed-case\">FAD<\/jats:styled-content> and promoted neuronal differentiation of human neural stem cells (<jats:styled-content style=\"fixed-case\">NSC<\/jats:styled-content>s) derived not only from fetal brain, but also from induced pluripotent stem cells. Depression of <jats:styled-content style=\"fixed-case\">FAD<\/jats:styled-content>\u2010dependent histone demethylase, lysine\u2010specific demethylase\u20101 (<jats:styled-content style=\"fixed-case\">LSD<\/jats:styled-content>1), prevented <jats:styled-content style=\"fixed-case\">FAD<\/jats:styled-content>\u2010induced neuronal differentiation. Furthermore, <jats:styled-content style=\"fixed-case\">FAD<\/jats:styled-content> influx facilitated nuclear localization of <jats:styled-content style=\"fixed-case\">LSD<\/jats:styled-content>1 and its enzymatic activity. Together, these findings led us to propose that <jats:styled-content style=\"fixed-case\">FAD<\/jats:styled-content> contributes to proper neuronal production from <jats:styled-content style=\"fixed-case\">NSC<\/jats:styled-content>s in the human fetal brain during development.<\/jats:p>","DOI":"10.1002\/2211-5463.12331","type":"journal-article","created":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T08:42:41Z","timestamp":1507279361000},"page":"1932-1942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<scp>FAD<\/scp> influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of <scp>LSD<\/scp>1"],"prefix":"10.1002","volume":"7","author":[{"given":"Kazumi","family":"Hirano","sequence":"first","affiliation":[{"name":"Molecular Neurophysiology Research Group Biomedical Research Institute The National Institute of Advanced Industrial Science and Technology (AIST) 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