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It has multiple interaction partners, with functions varying from the regulation of neuronal development and intracellular transport mechanisms to transcription regulation. One of its interactors is retinoic acid receptor (RAR), which is activated by retinoic acid and controls many target genes and physiological process. Based on previous evidence suggesting a possible nuclear role for FEZ1, we wanted to deepen our understanding of this function by addressing the FEZ1\u2013RAR interaction. We performed <jats:italic>in vitro<\/jats:italic> binding experiments and assessed the interface of interaction between both proteins. We found that FEZ1\u2013RAR interacted with a similar magnitude as RAR to its responsive element DR5 and that the interaction occurred in the coiled\u2010coil region of FEZ1 and in the ligand\u2010binding domain of RAR. Furthermore, cellular experiments were performed in order to confirm the interaction and screen for induced target genes from an 86\u2010gene panel. The analysis of gene expression showed that only in the presence of retinoic acid did FEZ1 induce <jats:italic>hoxb4<\/jats:italic> gene expression. This finding is consistent with data from the literature showing the <jats:italic>hoxb4<\/jats:italic> gene functionally involved in development and acute myeloid leukemia, as is FEZ1.<\/jats:p>","DOI":"10.1002\/2211-5463.12338","type":"journal-article","created":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T02:16:40Z","timestamp":1509589000000},"page":"4-14","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Fasciculation and elongation zeta\u20101 protein (FEZ1) interacts with the retinoic acid receptor and participates in transcriptional regulation of the <i>Hoxb4<\/i> gene"],"prefix":"10.1002","volume":"8","author":[{"given":"Mariana","family":"Bertini Teixeira","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina 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