{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T07:57:02Z","timestamp":1768982222895,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T00:00:00Z","timestamp":1510704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009483","name":"Institute of Global Innovation Research in TUAT","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009483","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>(\u2212)\u2010Epigallocatechin\u20103\u2010<jats:italic>O<\/jats:italic>\u2010gallate (<jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>), present in green tea, exhibits antioxidant and antiallergy effects. <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me, a 3\u2010<jats:italic>O<\/jats:italic>\u2010methylated derivative of <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>, has been reported to show similar biological functions; the inhibitory activity of <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me in a mouse allergy model was more potent than that of <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>, probably due to the efficiency of absorption from the intestine. However, the functional potency of these <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>s is controversial in each disease model. We previously observed that <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content> suppressed inflammatory bone resorption and prevented alveolar bone loss in a mouse model of periodontosis. In this study, we examined the role of <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me in bone resorption using a mouse model of periodontitis. Lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>)\u2010induced osteoclast formation was suppressed by adding <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me to cocultures of osteoblasts and bone marrow cells, and <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced bone resorption was also inhibited by <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me in calvarial organ cultures. <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me acted on osteoblasts and suppressed prostaglandin E (<jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>) production, which is critical for inflammatory bone resorption, by inhibiting the expression of <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">mPGES<\/jats:styled-content>\u20101, key enzymes for <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content> synthesis. In osteoclast precursor macrophages, <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me suppressed <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content>\u2010dependent differentiation into mature osteoclasts. In a mouse model of periodontitis, <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced bone resorption was suppressed by <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me in organ culture of mouse alveolar bone, and the alveolar bone loss was further attenuated by the treatment of <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me in the lower gingiva <jats:italic>in\u00a0vivo<\/jats:italic>. <jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>3\u2033Me may be a potential natural compound for the protection of inflammatory bone loss in periodontitis.<\/jats:p>","DOI":"10.1002\/2211-5463.12340","type":"journal-article","created":{"date-parts":[[2017,11,4]],"date-time":"2017-11-04T09:22:38Z","timestamp":1509787358000},"page":"1972-1981","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Effects of <i>O<\/i>\u2010methylated (\u2212)\u2010epigallocatechin gallate (<scp>EGCG<\/scp>) on <scp>LPS<\/scp>\u2010induced osteoclastogenesis, bone resorption, and alveolar bone loss in mice"],"prefix":"10.1002","volume":"7","author":[{"given":"Tsukasa","family":"Tominari","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryota","family":"Ichimaru","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shosei","family":"Yoshinouchi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiho","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenta","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Institute of Global Innovation Research Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian M.W.","family":"Grundler","sequence":"additional","affiliation":[{"name":"Institute of Crop Science and Resource Conservation University of Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Inada","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"},{"name":"Institute of Global Innovation Research Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chisato","family":"Miyaura","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Life Science Tokyo University of Agriculture and Technology Koganei Japan"},{"name":"Institute of Global Innovation Research Tokyo University of Agriculture and Technology Koganei Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.10.079"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxq431"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.2504"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3597"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81569-X"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.01.146"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1879"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002079200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.4.7405"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01432-7"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510378682"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf204497b"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000313d"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf071176o"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2015.06.003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2144\/05391RV01"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-2840"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb743"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.09.095"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf904335g"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b13-00349"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3136\/nskkk.56.403"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.755959"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-012-0485-z"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.150612"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090031"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5293"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414185200"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107897200"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.10.1885"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12071"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F2211-5463.12340","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T12:15:39Z","timestamp":1695989739000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,15]]},"references-count":31,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1002\/2211-5463.12340"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12340","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,15]]},"assertion":[{"value":"2017-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}