{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T17:53:38Z","timestamp":1776880418145,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:00:00Z","timestamp":1539043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Margareta af Ugglas Foundation"},{"name":"Foundation for Geriatric Diseases at Karolinska Institutet"},{"name":"Loo & Hans Osterman Foundation"},{"name":"KI Foundations"},{"name":"Lindh\u00e9s Advokatbyr\u00e5 AB Foundation"},{"name":"Gunvor and Josef An\u00e9rs Foundation"},{"name":"the Swedish Brain Foundation"},{"name":"Magnus Bergvalls Foundation"},{"DOI":"10.13039\/100009673","name":"Gun and Bertil Stohnes Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009673","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tore Nilssons Foundation"},{"DOI":"10.13039\/100010815","name":"Foundation for Old Servants","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010815","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"crossref","award":["2015-02774"],"award-info":[{"award-number":["2015-02774"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>Dicarboxylate clamp tetratricopeptide repeat (dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content>) motif\u2010containing proteins are well\u2010known partners of the heat shock protein (Hsp) 70 and Hsp90 molecular chaperones. Together, they facilitate a variety of intracellular processes, including protein folding and maturation, protein targeting, and protein degradation. An extreme C\u2010terminal sequence, the EEVD motif, is identical in Hsp70 and Hsp90, and is indispensable for their interaction with dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content> proteins. However, almost no information is available on the existence of other potential dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content>\u2010interacting proteins. We searched the human protein database for proteins with C\u2010terminal sequences similar to that of Hsp70\/Hsp90 to identify potential partners of dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content> proteins. The search identified 112 proteins containing a Hsp70\/Hsp90\u2010like signature at their C termini. Gene Ontology enrichment analysis of identified proteins revealed enrichment of distinct protein classes, such as molecular chaperones and proteins of the ubiquitin\u2013proteasome system, highlighting the possibility of functional specialization of proteins containing a Hsp70\/Hsp90\u2010like signature. We confirmed interactions of selected proteins containing Hsp70\/Hsp90\u2010like C termini with dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content> proteins both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0situ<\/jats:italic>. Analysis of interactions of 10\u2010amino\u2010acid peptides corresponding to the C termini of identified proteins with dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content> proteins revealed significant differences in binding strength between various peptides. We propose a hierarchical mode of interaction within the dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content> protein network. These findings describe a novel dc<jats:styled-content style=\"fixed-case\">TPR<\/jats:styled-content> protein interaction networks and provide a rationale for selective regulation of protein\u2013protein interactions within this network.<\/jats:p>","DOI":"10.1002\/2211-5463.12521","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T10:34:33Z","timestamp":1536575673000},"page":"1830-1843","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>In silico<\/i> identification and biochemical characterization of the human dicarboxylate clamp <scp>TPR<\/scp> protein interaction network"],"prefix":"10.1002","volume":"8","author":[{"given":"Alexandra","family":"Bernadotte","sequence":"first","affiliation":[{"name":"Department of Molecular Biochemistry and Biophysics Karolinska Institutet Solna Sweden"},{"name":"Faculty of Mechanics and Mathematics Lomonosov Moscow State University Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajnish","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Neurogeriatrics Department of Neuroscience Care and Society Karolinska Institutet Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Winblad","sequence":"additional","affiliation":[{"name":"Division of Neurogeriatrics Department of Neuroscience Care and Society Karolinska Institutet Huddinge Sweden"},{"name":"Memory Clinic Theme Aging Karolinska University Hospital Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel F.","family":"Pavlov","sequence":"additional","affiliation":[{"name":"Division of Neurogeriatrics Department of Neuroscience Care and Society Karolinska Institutet Huddinge Sweden"},{"name":"Memory Clinic Theme Aging Karolinska University Hospital Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2003.08.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80830-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2003.10.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.05.019"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.05.039"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.280446"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200305051"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.22.7569-7575.2001"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36622"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090543"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2017.00185"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467208666150519114115"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467208666150519113639"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201600050"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2016.02.005"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2011.11.006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-48"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.0030039"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021800"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-14731-z"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2012.08.012"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11491"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01891-08"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth947"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309814200"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-04-0319"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2013.12.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.03.002"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147515"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04716"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100531200"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005889200"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02169.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.13.8570"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108312200"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F2211-5463.12521","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12521","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.12521","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12521","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T01:07:13Z","timestamp":1694826433000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12521"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,9]]},"references-count":35,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/2211-5463.12521"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12521","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,9]]},"assertion":[{"value":"2018-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}