{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T20:15:34Z","timestamp":1783541734854,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,5]],"date-time":"2019-03-05T00:00:00Z","timestamp":1551744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/22452\u20101"],"award-info":[{"award-number":["2017\/22452\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:label\/><jats:p>Leishmaniasis is a neglected disease caused by a trypanosomatid protozoan of the genus <jats:italic>Leishmania<\/jats:italic>. Most drugs used to treat leishmaniasis are highly toxic, and the emergence of drug\u2010resistant strains has been observed. Therefore, new therapeutic targets against leishmaniasis are required. Several isoprenoid compounds, including dolichols or ubiquinones, have been shown to be important for cell viability and proliferation in various trypanosomatid species. Here, we detected the biosynthesis of tocopherol in <jats:italic>Leishmania<\/jats:italic> (<jats:italic>L<\/jats:italic>.) <jats:italic>amazonensis<\/jats:italic> promastigotes <jats:italic>in\u00a0vitro<\/jats:italic> through metabolic labelling with [1\u2010(n)\u20103H]\u2010phytol. Subsequently, we confirmed the presence of vitamin E in the parasite by gas chromatography\u2013mass spectrometry. Treatment with usnic acid or nitisinone, inhibitors of precursors of vitamin E synthesis, inhibited growth of the parasite in a concentration\u2010dependent manner. This study provides the first evidence of tocopherol biosynthesis in a trypanosomatid and suggests that inhibitors of the enzyme 4\u2010hydroxyphenylpyruvate dioxygenase may be suitable for use as antileishmanial compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Database<\/jats:title><jats:p>The amino acid sequence of a conserved hypothetical protein [Leishmania mexicana MHOM\/GT\/2001\/U1103] has been deposited in GenBank (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"CBZ28005.1\">CBZ28005.1<\/jats:ext-link>)<\/jats:p><\/jats:sec>","DOI":"10.1002\/2211-5463.12613","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T03:13:30Z","timestamp":1551150810000},"page":"743-754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Tocopherol biosynthesis in <i>Leishmania<\/i> (<i>L<\/i>.) <i>amazonensis<\/i> promastigotes"],"prefix":"10.1002","volume":"9","author":[{"given":"Jos\u00e9 M\u00e1rio F.","family":"Balanco","sequence":"first","affiliation":[{"name":"Department of Parasitology Institute of Biomedical Sciences University of S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo A.C.","family":"Sussmann","sequence":"additional","affiliation":[{"name":"Department of Parasitology Institute of Biomedical Sciences University of S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignasi B.","family":"Verdaguer","sequence":"additional","affiliation":[{"name":"Department of Parasitology Institute of Biomedical Sciences University of S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heloisa B.","family":"Gabriel","sequence":"additional","affiliation":[{"name":"Department of Parasitology Institute of Biomedical Sciences University of S\u00e3o Paulo 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