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Recent studies have suggested that cerebral ischemia\u2010induced neurogenesis plays a vital role in post\u2010stroke repair. Overactivation of <jats:styled-content style=\"fixed-case\">AMP<\/jats:styled-content>\u2010activated protein kinase (<jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content>), a master sensor of energy balance, has been reported to exacerbate neuron apoptosis, but the role of chronic <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> stimulus in post\u2010stroke recovery remains unclear. Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s have emerged as regulators of neurogenesis and have been reported to be involved in neurological function. In this study, we verified that miR\u201027b directly targets <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> and inhibits <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> expression. In cultured neural stem cells, miR\u201027b inhibitor improved proliferation and differentiation via the <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> signaling pathway, but did not have an obvious effect on cell viability under oxygen and glucose deprivation conditions. In a mouse middle cerebral artery occlusion model, administration of miR\u201027b inhibitor significantly enhanced behavioral function recovery and spatial memory. Up\u2010regulation of neurogenesis was observed both in the subventricular zone and in the hippocampal dentate gyrus. Collectively, our data suggest that miR\u201027b inhibition promotes recovery after ischemic stroke by regulating <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> activity. These findings may facilitate the development of novel therapeutic strategies for stroke.<\/jats:p>","DOI":"10.1002\/2211-5463.12614","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T06:09:15Z","timestamp":1551161355000},"page":"859-869","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Inhibition of mi<scp>RNA<\/scp>\u201027b enhances neurogenesis via <scp>AMPK<\/scp> activation in a mouse ischemic stroke model"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9496-4027","authenticated-orcid":false,"given":"Zhengang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Affiliated Hospital of Weifang Medical University China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Affiliated Hospital of 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