{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T12:17:01Z","timestamp":1770121021884,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 12680607"],"award-info":[{"award-number":["JP 12680607"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Liver cirrhosis (<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>) is a disease characterized by pathological accumulation and alteration of extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>) proteins; the interaction between two such proteins, collagen and vitronectin (<jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>), is considered to be the key to controlling <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> remodeling in liver cirrhosis. If it is possible to control the modification of oligosaccharides on <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>, it may be possible to retard progression of liver cirrhosis. In this study, we examined the relationship between changes in <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> glycosylation and activity related to the remodeling of hepatic tissue in human <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> and a rat model of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> generated using carbon tetrachloride (<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l<jats:sub>4<\/jats:sub>). Plasma concentrations of <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> in human <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> declined to approximately two\u2010thirds that in normal plasma, but the ratio of active <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>, which has collagen\u2010binding activities, increased 2.8 times in <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content> plasma. In contrast, purified <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> exhibited similar binding activities toward type I, <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>, and V collagens to those of normal <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>. Lectin reactivities and carbohydrate analyses of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> revealed that branching, fucosylation, and sialylation of <jats:italic>N<\/jats:italic>\u2010glycans were higher than those of normal <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>. On the other hand, the plasma level of rat <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l<jats:sub>4<\/jats:sub>\u2010<jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> increased and the ratio of active molecules to collagen in plasma decreased. Increased fucosylation of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> was not detected in carbohydrates of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l<jats:sub>4<\/jats:sub>\u2010<jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>. The changes in rat <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> due to <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l<jats:sub>4<\/jats:sub> treatment did not correspond to the changes in plasma levels of human <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> caused by <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>, the ratio of active molecules, or carbohydrate composition, thereby indicating that <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l<jats:sub>4<\/jats:sub>\u2010treated rats are not an appropriate model for studying <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>s in human <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>. Glycosidase treatment of <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content>s supported the hypothesis that the collagen\u2010binding activity of <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> is modulated by alterations of glycosylation during <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>, which may contribute to (a) the matrix incorporation of <jats:styled-content style=\"fixed-case\">VN<\/jats:styled-content> and (b) tissue fibrosis.<\/jats:p>","DOI":"10.1002\/2211-5463.12616","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T04:13:28Z","timestamp":1551500008000},"page":"755-768","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vitronectins produced by human cirrhotic liver and <scp>CC<\/scp>l<sub>4<\/sub>\u2010treated rats differ in their glycosylation pattern and tissue remodeling 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