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Centrins are associated with the microtubule\u2010organizing center and with centrosome\u2010related structures, such as basal bodies in flagellar and ciliated cells, and the spindle pole body in yeast. Five centrin genes have been identified in <jats:italic>Trypanosoma\u00a0brucei<\/jats:italic> (<jats:italic>T.\u00a0brucei<\/jats:italic>), a protozoan parasite that causes sleeping sickness in humans and nagana in cattle in sub\u2010Saharan Africa. In the present study, we identified that centrin5 of <jats:italic>T.\u00a0brucei<\/jats:italic> (TbCentrin5) is localized throughout the cytosol and nucleus and enriched in the flagellum. We further identified that TbCentrin5 binds Ca<jats:sup>2+<\/jats:sup> ions with a high affinity and constructed a model of TbCentrin5 bound by Ca<jats:sup>2+<\/jats:sup> ions. Meanwhile, we observed that TbCentrin5 interacts with TbCentrin1, TbCentrin3, and TbCentrin4 and that the interactions are Ca<jats:sup>2+<\/jats:sup>\u2010dependent, suggesting that TbCentrin5 is able to form different complexes with other TbCentrins to participate in relevant cellular processes. Our study provides a foundation for better understanding of the biological roles of TbCentrin5.<\/jats:p>","DOI":"10.1002\/2211-5463.12683","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T09:36:14Z","timestamp":1559640974000},"page":"1421-1431","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Trypanosoma\u00a0brucei<\/i> centrin5 is enriched in the flagellum and interacts with other centrins in a calcium\u2010dependent manner"],"prefix":"10.1002","volume":"9","author":[{"given":"Fangzhen","family":"Shan","sequence":"first","affiliation":[{"name":"Hefei National Laboratory for Physical Science at Microscale and School of Life Science University of Science and Technology of China Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Hefei National Laboratory for Physical Science at 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