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In the current study, we explored the impact of <jats:italic>S<\/jats:italic>\u2010allyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine (<jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content>), an organosulfur component of garlic, on hepatocyte apoptosis induced by alcohol. Rat liver (<jats:styled-content style=\"fixed-case\">BRL<\/jats:styled-content>\u20103A) cells were challenged by ethanol with or without <jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content> treatment. Cell death\/viability, reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) generation, mitochondrial Cytochrome C release, and caspase 3 activity were then examined. We found that ethanol remarkably induced apoptosis of hepatocytes, while <jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content> treatment rescued ethanol\u2010induced hepatocyte injury, as demonstrated by cell counting kit\u20108 (<jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8) assay, <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> assay, and annexin V\/<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> staining assay. Ethanol evoked <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> generation in <jats:styled-content style=\"fixed-case\">BRL<\/jats:styled-content>\u20103A cells, and this was abated by <jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content> pretreatment, as indicated by 2\u2032,7\u2032\u2010dichlorofluorescin diacetate (<jats:styled-content style=\"fixed-case\">DCFDA<\/jats:styled-content>) staining assay. Moreover, ethanol suppressed cellular anti\u2010apoptotic protein B\u2010cell lymphoma\u20102 (Bcl\u20102) expression, increased pro\u2010apoptotic protein Bcl\u20102\u2010associated X protein (Bax) expression, induced mitochondrial Cytochrome C release, and activated the caspase 3\u2010dependent apoptosis pathway in <jats:styled-content style=\"fixed-case\">BRL<\/jats:styled-content>\u20103A cells. <jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content> was sufficient to abolish all these changes induced by ethanol, thereby revealing the molecular mechanisms underlying its protective effects. In conclusion, <jats:styled-content style=\"fixed-case\">SAC<\/jats:styled-content> protects hepatocytes from ethanol\u2010induced apoptosis and may be suitable for use as a novel anti\u2010apoptotic agent for treating <jats:styled-content style=\"fixed-case\">ARLD<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/2211-5463.12684","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T09:39:18Z","timestamp":1559641158000},"page":"1327-1336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<i>S<\/i>\u2010allyl\u2010<scp>l<\/scp>\u2010cysteine (SAC) protects hepatocytes from alcohol\u2010induced apoptosis"],"prefix":"10.1002","volume":"9","author":[{"given":"Peng","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery the Second Affiliated Hospital of Kunming Medical University China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingdao","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery the Second Affiliated Hospital of Kunming Medical University China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery the Second Affiliated Hospital of Kunming Medical University China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henghai","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery the Second Affiliated Hospital of Kunming Medical University China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6741-7036","authenticated-orcid":false,"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology the Second People's Hospital of Yunnan Province Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.041"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.04.029"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/740149"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.03.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2013.819428"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2007.05.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000046892"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(83)90482-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1127"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.04.021"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.499"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/907162"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2011.1922"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.07145.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986711796504664"},{"key":"e_1_2_9_17_1","first-page":"439","article-title":"S\u2010allylcysteine induces cell cycle arrest and apoptosis in androgen\u2010independent human prostate cancer cells","volume":"5","author":"Liu Z","year":"2012","journal-title":"Mol Med Rep"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031655"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2012.02.015"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12986"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.08.052"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00658"},{"key":"e_1_2_9_23_1","first-page":"39","article-title":"Quinotrierixin inhibits proliferation of human retinal pigment epithelial cells","volume":"19","author":"Chen C","year":"2013","journal-title":"Mol Vision"},{"key":"e_1_2_9_24_1","first-page":"21","article-title":"TUNEL assay. 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