{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T13:39:22Z","timestamp":1778938762838,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,10]],"date-time":"2019-08-10T00:00:00Z","timestamp":1565395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p><jats:italic>Helicobacter\u00a0pylori<\/jats:italic> infection (<jats:styled-content style=\"fixed-case\">HPI<\/jats:styled-content>) is a prevalent infectious disease associated with gastric ulcer, gastric cancer, and many nongastrointestinal disorders. To identify genes that may serve as microbial markers for <jats:styled-content style=\"fixed-case\">HPI<\/jats:styled-content>, we performed shotgun metagenomic sequencing of fecal samples from 313 Chinese volunteers who had undergone a C14 breath test. Through comparing differences in intestinal microbial community structure between <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010positive and <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010negative individuals, we identified 58 <jats:styled-content style=\"fixed-case\">HPI<\/jats:styled-content>\u2010associated microbial species (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05, Wilcoxon test). A classifier based on microbial species markers showed high diagnostic ability for <jats:styled-content style=\"fixed-case\">HPI<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>\u00a0=\u00a00.84). Furthermore, levels of gut microbial vitamin B12 (<jats:styled-content style=\"fixed-case\">VB<\/jats:styled-content>12) biosynthesis and plasma <jats:styled-content style=\"fixed-case\">VB<\/jats:styled-content>12 were significantly lower in <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010positive individuals compared with <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010negative individuals (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05, Wilcoxon test). This study reveals that certain alterations in gut microbial species and functions are associated with <jats:styled-content style=\"fixed-case\">HPI<\/jats:styled-content> and shows that gut microbial shift in <jats:styled-content style=\"fixed-case\">HPI<\/jats:styled-content> patients may indirectly elevate the risk of <jats:styled-content style=\"fixed-case\">VB<\/jats:styled-content>12 deficiency.<\/jats:p>","DOI":"10.1002\/2211-5463.12694","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T09:27:52Z","timestamp":1561714072000},"page":"1552-1560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Alterations in the human gut microbiome associated with <i>Helicobacter\u00a0pylori<\/i> infection"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-8527","authenticated-orcid":false,"given":"Daoming","family":"Wang","sequence":"first","affiliation":[{"name":"School of Future Technology University of Chinese Academy of Sciences Beijing China"},{"name":"BGI\u2010Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanzi","family":"Zhong","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"},{"name":"China National GeneBank BGI\u2010Shenzhen China"},{"name":"Laboratory of Genomics and Molecular Biomedicine Department of Biology Copenhagen Biocenter University of Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuxia","family":"Ding","sequence":"additional","affiliation":[{"name":"iCarbonX Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxiang","family":"Lin","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"},{"name":"China National GeneBank BGI\u2010Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanmei","family":"Tang","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"},{"name":"China National GeneBank BGI\u2010Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zong","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Future Technology University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanming","family":"Yang","sequence":"additional","affiliation":[{"name":"BGI\u2010Shenzhen China"},{"name":"James D. 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