{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:43:21Z","timestamp":1784040201969,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,9,29]],"date-time":"2019-09-29T00:00:00Z","timestamp":1569715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271736"],"award-info":[{"award-number":["81271736"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2019,11]]},"abstract":"RNA<\/jats:styled-content>\u2010sequencing (RNA<\/jats:styled-content>\u2010Seq) is a useful method to explore the molecular events in cells and tissues at the transcriptional level. However, comprehensive transcriptome analysis of hepatocarcinogenesis and progression is lacking. In this study, we aimed to characterize a dimethylnitrosamine (DEN<\/jats:styled-content>) and carbon tetrachloride (CC<\/jats:styled-content>l4<\/jats:sub>; DEN<\/jats:styled-content>+CC<\/jats:styled-content>l4<\/jats:sub>)\u2010induced hepatocellular carcinoma (HCC<\/jats:styled-content>) mouse model by RNA<\/jats:styled-content>\u2010Seq. In total, 2033 genes were up\u2010regulated and 841 genes were down\u2010regulated after DEN<\/jats:styled-content> and CC<\/jats:styled-content>l4<\/jats:sub> stimulation. The differentially expressed genes were highly enriched for the Gene Ontology terms oxoacid metabolic process, carboxylic acid metabolic process, and organic acid metabolic process. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the top five significantly overrepresented pathways were metabolic pathways, chemical carcinogenesis, steroid hormone biosynthesis, retinol metabolism and metabolism of xenobiotics by cytochrome P450. Moreover, a protein\u2010protein interaction network analysis indicated that Rous sarcoma oncogene (Src) may play a key role in DEN<\/jats:styled-content>+CC<\/jats:styled-content>l4<\/jats:sub>\u2010induced HCC<\/jats:styled-content>. These results provide a comprehensive overview of transcriptome events in DEN<\/jats:styled-content>+CC<\/jats:styled-content>l4<\/jats:sub>\u2010induced HCC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/2211-5463.12724","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T12:54:31Z","timestamp":1566392071000},"page":"1900-1908","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Whole transcriptome analysis of chemically induced hepatocellular carcinoma using RNA<\/scp>\u2010sequencing analysis"],"prefix":"10.1002","volume":"9","author":[{"given":"Jie","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hygiene Inspection and Quarantine School of Public Health Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3273-5760","authenticated-orcid":false,"given":"Derun","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Anhui Medical University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30010-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1401965111"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28610"},{"key":"e_1_2_9_6_1","first-page":"41","article-title":"Molecular and cellular oncogenic mechanisms in hepatocellular carcinoma","volume":"108","author":"Setshedi M","year":"2018","journal-title":"S Afr Med J"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230252824770"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2012-111793"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078644"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2484"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158441"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1226"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1613"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14421"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.05.015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312270"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.227181.117"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.169"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F2211-5463.12724","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12724","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.12724","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12724","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T14:50:58Z","timestamp":1694184658000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12724"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,29]]},"references-count":17,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/2211-5463.12724"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12724","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,29]]},"assertion":[{"value":"2019-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}