{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T05:10:05Z","timestamp":1733548205180,"version":"3.30.1"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,11,17]],"date-time":"2019-11-17T00:00:00Z","timestamp":1573948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>Cervical squamous cell carcinoma (CSCC) accounts for a significant proportion of cervical cancer; thus, there is a need for novel and noninvasive diagnostic biomarkers for this malignancy. In this study, we performed integrated analysis of a dataset from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) and differentially expressed miRNAs (DEmiRNAs) between CSCC, cervical intraepithelial neoplasia (CIN) and healthy control subjects. We further established protein\u2010protein interaction and DEmiRNA\u2010target gene interaction networks, and performed functional annotation of the target genes of DEmiRNAs. In total, we identified 1375 DEGs and 19 DEmiRNAs in CIN versus normal control, and 2235 DEGs and 33 DEmiRNAs in CSCC versus CIN by integrated analysis. Our protein\u2010protein interaction network indicates that the common DEGs, <jats:italic>Cyclin B\/cyclin\u2010dependent kinase 1<\/jats:italic> (<jats:italic>CDK1<\/jats:italic>), <jats:italic>CCND1<\/jats:italic>, <jats:italic>ESR1<\/jats:italic> and <jats:italic>Aurora kinase A<\/jats:italic> (<jats:italic>AURKA<\/jats:italic>), are the top four hub genes. P53 and prostate cancer were identified as significantly enriched signaling pathways of common DEGs and DEmiRNA targets, respectively. We validated that expression levels of three DEGs (<jats:italic>TYMS<\/jats:italic>, <jats:italic>SASH1<\/jats:italic> and <jats:italic>CDK1<\/jats:italic>) and one DEmiRNA of hsa\u2010miR\u201099a were altered in blood samples of patients with CSCC. In conclusion, a total of four DEGs (<jats:italic>TYMS<\/jats:italic>, <jats:italic>SASH1<\/jats:italic>, <jats:italic>CDK1<\/jats:italic> and <jats:italic>AURKA<\/jats:italic>) and two DEmiRNAs (hsa\u2010miR\u201021 and hsa\u2010miR\u201099a) may be involved in the pathogenesis of CIN and the progression of CIN into CSCC. Of these, <jats:italic>TYMS<\/jats:italic> is predicted to be regulated by hsa\u2010miR\u201099a and <jats:italic>SASH1<\/jats:italic> to be regulated by hsa\u2010miR\u201021.<\/jats:p>","DOI":"10.1002\/2211-5463.12747","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T19:08:06Z","timestamp":1571857686000},"page":"2080-2092","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prediction of a miRNA\u2010mRNA functional synergistic network for cervical squamous cell carcinoma"],"prefix":"10.1002","volume":"9","author":[{"given":"Dan","family":"Sun","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Dalian Maternity and Child Health Care Hospital 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