{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:28:04Z","timestamp":1790749684262,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T00:00:00Z","timestamp":1584144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>\n                    Type VII collagen is an extracellular matrix protein, which is important for skin stability; however, detailed information at the molecular level is scarce. The second vWFA (von Willebrand factor type A) domain of type VII collagen mediates important interactions, and immunization of mice induces skin blistering in certain strains. To understand vWFA2 function and the pathophysiological mechanisms leading to skin blistering, we structurally characterized this domain by X\u2010ray crystallography and NMR spectroscopy. Cell adhesion assays identified two new interactions: one with \u03b21 integrin via its RGD motif and one with laminin\u2010332. The latter interaction was confirmed by surface plasmon resonance with a\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    of about 1\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    . These data show that vWFA2 has additional functions in the extracellular matrix besides interacting with type I collagen.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.12807","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T10:08:35Z","timestamp":1581070115000},"page":"580-592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3989-4748","authenticated-orcid":false,"given":"Jan M.","family":"Gebauer","sequence":"first","affiliation":[{"name":"Institute of Biochemistry University of Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7051-8719","authenticated-orcid":false,"given":"Florian","family":"Flachsenberg","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Metabolomics University of L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cordula","family":"Windler","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Metabolomics University of L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Richer","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Metabolomics University of L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Baumann","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry University of Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9885-0315","authenticated-orcid":false,"given":"Karsten","family":"Seeger","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Metabolomics University of L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2011.03.011"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13446"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.531327"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38784-8"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2014.04.006"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/1.7.475"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.03.034"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00491.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.14516"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802415200"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.11.119"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2008.00723.x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/812029"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2011.606450"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00603"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2011.606451"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.248"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-8-93"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300310"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.04.071"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0909049512009715"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047337"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913000061"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1107\/S090744491003982X"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905036693"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2013.06.028"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.91"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911001314"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360221211"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1105"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnmrs.2010.12.003"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20449"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja001068h"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmre.1998.1361"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.36"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0000908"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.761"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2017.05.001"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2009.07.006"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja9812610"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2005.08.006"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.051844006"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.073395108"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.23188"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4473"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310424200"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1106209"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw357"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069833"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1385"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4392"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI68127"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F2211-5463.12807","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12807","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.12807","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12807","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T03:56:26Z","timestamp":1768190186000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12807"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,14]]},"references-count":55,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/2211-5463.12807"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12807","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,14]]},"assertion":[{"value":"2019-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}