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During the pathological process, acid\u2010sensing ion channel 1a (ASIC1a), which is responsible for Ca<jats:sup>2+<\/jats:sup> transportation, is involved in the activation of HSCs. It has previously been identified that ASIC1a is related to pyroptosis in articular chondrocytes. However, it remains unclear whether ASIC1a restrains pyroptosis during liver fibrosis. Here, we determined that the levels of pyroptosis\u2010associated speck\u2010like protein, gasdermin D, caspase\u20101, nucleotide\u2010binding oligomerization domain (NOD)\u2010like receptor 3, and apoptosis\u2010associated speck\u2010like protein (ASC) decreased, while the level of \u03b1\u2010smooth muscle actin and collagen\u2010I increased upon introduction of ASIC1a into an acid\u2010induced model. Inhibition or silencing of ASIC1a and the use of Ca<jats:sup>2+<\/jats:sup>\u2010free medium were able to promote the pyroptosis of activated HSCs, which reduced their deposition. In summary, our study indicates that ASIC1a inhibits pyroptosis of HSCs and that inhibition of ASIC1a may be able to promote pyroptosis to relieve liver fibrosis.<\/jats:p>","DOI":"10.1002\/2211-5463.12850","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T04:17:26Z","timestamp":1585714646000},"page":"1044-1055","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["ASIC1a inhibits cell pyroptosis induced by acid\u2010induced activation of rat hepatic stellate cells"],"prefix":"10.1002","volume":"10","author":[{"given":"Jun","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmacy Anhui Medical University Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingjin","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui Medical University Hefei 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