{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:24:45Z","timestamp":1793661885196,"version":"4.5.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T00:00:00Z","timestamp":1588118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"crossref","award":["2016YFC1000503"],"award-info":[{"award-number":["2016YFC1000503"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p>Although 19p13.13 microdeletion syndrome has been consistently associated with intellectual disability, overgrowth, and macrocephaly, the underlying mechanisms remain unclear. MAST1, a member of the microtubule\u2010associated serine\/threonine kinase family, has been suggested as a potential candidate gene responsible for neurologic abnormalities in 19p13.13 microdeletion syndrome, but its role in nervous system development remains to be elucidated. Here, we investigated how MAST1 contributes to neuronal development. We report that MAST1 is upregulated during neuronal differentiation of the human neuroblastoma cell line, SH\u2010SY5Y. Inhibition of MAST1 expression by RNA interference attenuated neuronal differentiation of SH\u2010SY5Y cells. Cell cycle analyses revealed that MAST1\u2010depleted cells did not undergo cell cycle arrest after RA treatment. Consistent with this observation, the number of EdU\u2010positive cells significantly increased in MAST1 knockdown cells. Intriguingly, levels of P27, a cyclin\u2010dependent kinase inhibitor, were also increased during neuronal differentiation, and MAST1 knockdown reduced the expression of P27. Moreover, reduced neuronal differentiation caused by MAST1 depletion was rescued partially by P27 overexpression in SH\u2010SY5Y cells. Collectively, these results suggest that MAST1 influences nervous system development by affecting neuronal differentiation through P27.<\/jats:p>","DOI":"10.1002\/2211-5463.12860","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T08:26:30Z","timestamp":1586939190000},"page":"1104-1114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["MAST1 modulates neuronal differentiation and cell cycle exit via P27 in neuroblastoma cells"],"prefix":"10.1002","volume":"10","author":[{"given":"Tianrui","family":"Jing","sequence":"first","affiliation":[{"name":"Key Laboratory of Metabolism and Molecular Medicine Ministry of Education Department of Biochemistry and Molecular Biology School of Basic Medical Sciences &amp; Institutes of Biomedical Sciences Shanghai Medical College Fudan University Shanghai 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