{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T04:34:21Z","timestamp":1769834061102,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T00:00:00Z","timestamp":1590883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:p>Osteosarcoma is a malignant bone tumor, and clinically detectable metastases can be detected in ~\u00a015\u201320% of patients when they seek medical advice; patients with metastatic disease have extremely poor prognosis. Here, we examined the involvement of the microRNA miR\u2010505 in osteosarcoma. Eighty\u2010four patients seeking treatment for osteosarcoma were included in the study group (SG), and 63 healthy subjects were allocated to the control group (CG). Normal human bone cells MG\u201063 and U20S cells were transfected with miR\u2010505 mimics, miR\u2010NC, HMGB1 RNA for targeted inhibition (si\u2010HMGB1), and si\u2010NC to examine the effects on HMGB1 expression. Cell proliferation, invasion, and apoptosis were measured using CCK\u20108, scratch assays, and flow cytometry (FCM), respectively, and the relationship between miR\u2010505 and HMGB1 was determined using the dual\u2010luciferase reporter assay. In patient tissues and serum, miR\u2010505 was expressed at a low level, and HMGB1 was expressed at a high level, with an area under curve of &gt;\u00a00.9. Furthermore, the expression of miR\u2010505 and HMGB1 in tissues had a positive association with that in the serum, whereas the expression of miR\u2010505 had a negative association with that of HMGB1 in tissues only. Overexpression of miR\u2010505 and silencing of HMGB1 suppressed the proliferation, migration, and invasion of osteosarcoma cells and increased the rate of apoptosis, whereas the co\u2010transfected miR\u2010505 mimics\u00a0+\u00a0si\u2010HMGB1 demonstrated a more significant inhibitory effect on the proliferation and invasion of osteosarcoma cells and a higher apoptosis rate. miR\u2010505 may inhibit the proliferation and invasion and promote apoptosis of osteosarcoma cells by targeting and suppressing HMGB1.<\/jats:p>","DOI":"10.1002\/2211-5463.12868","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T17:24:35Z","timestamp":1588181075000},"page":"1251-1260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["miR\u2010505 inhibits proliferation of osteosarcoma via 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