{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:17:24Z","timestamp":1786976244446,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T00:00:00Z","timestamp":1593129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p>\n                    Osteoporosis is characterized by systemic microarchitecture impairment and bone loss, which ultimately lead to fragility fractures. This disease is most common in older people, especially in postmenopausal women. Cancellous bone is affected by osteoporosis earlier than cortical bone, and DNA methylation microarray analysis of the hip cancellous bone of patients with osteoarthritis revealed differential methylation. In view of the important role of cancellous bone in bone development, we examined genome\u2010wide DNA methylation profiles in the cancellous bone from patients with postmenopausal osteoporosis versus healthy postmenopausal women using Illumina 850K methylation microarray analysis. Under a threshold of\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05, we obtained a total of 8973 differentially methylated genes, such as\n                    <jats:italic>SOX6<\/jats:italic>\n                    ,\n                    <jats:italic>ACE<\/jats:italic>\n                    ,\n                    <jats:italic>SYK<\/jats:italic>\n                    and\n                    <jats:italic>TGFB3<\/jats:italic>\n                    . Under a threshold of\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05 and |\u25b3\u03b2| &gt; 0.2, a total of 17 and 34 key differentially methylated genes were further identified at the promoter region and cytosine\u2010 phosphate\u2010 guanine (CpG) islands (such as\n                    <jats:italic>PRKCZ<\/jats:italic>\n                    ,\n                    <jats:italic>GNA11<\/jats:italic>\n                    and\n                    <jats:italic>COL4A1<\/jats:italic>\n                    ), respectively.\n                    <jats:italic>PLEKHA2<\/jats:italic>\n                    ,\n                    <jats:italic>PLEKHB1<\/jats:italic>\n                    ,\n                    <jats:italic>PNPLA7<\/jats:italic>\n                    ,\n                    <jats:italic>SCD<\/jats:italic>\n                    ,\n                    <jats:italic>MGST3<\/jats:italic>\n                    and\n                    <jats:italic>TSNAX<\/jats:italic>\n                    were the most common differentially methylated genes at both the promoter region and CpG islands. Five important signaling pathways, including the calcium signaling pathway, the cyclic guanosine phospho\u2010protein kinase G (cGMP\u2010PKG) signaling pathway, endocytosis, the Rap1 signaling pathway and the AMPK signaling pathway were identified. Our study may be suitable as a basis for exploring the mechanisms underlying osteoporosis in postmenopausal women.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.12907","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T05:34:43Z","timestamp":1591248883000},"page":"1516-1531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Alterations in DNA methylation profiles in cancellous bone of postmenopausal women with osteoporosis"],"prefix":"10.1002","volume":"10","author":[{"given":"Yu","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Orthopedics People\u2019s Hospital of Deyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics People\u2019s Hospital of Deyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics People\u2019s Hospital of Deyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics People\u2019s Hospital of Deyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics People\u2019s Hospital of Deyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics People\u2019s Hospital of Deyang City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics 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