{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:00:34Z","timestamp":1747144834335,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000835","name":"University of Bath","doi-asserted-by":"publisher","award":["\u2010"],"award-info":[{"award-number":["\u2010"]}],"id":[{"id":"10.13039\/501100000835","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p><jats:italic>Clostridium botulinum<\/jats:italic> neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcholine release from motor neurons; however, at tiny doses, this property is exploited for use as a therapeutic. Each member of the BoNT family of proteins consists of three distinct domains: a binding domain that targets neuronal cell membranes (H<jats:sub>C<\/jats:sub>), a translocation domain (H<jats:sub>N<\/jats:sub>) and a catalytic domain (LC). Here, we present high\u2010resolution crystal structures of the binding domains of BoNT subtypes\/A5 (H<jats:sub>C<\/jats:sub>\/A5) and\/A6 (H<jats:sub>C<\/jats:sub>\/A6). These structures show that the core fold identified in other subtypes is maintained, but with subtle differences at the expected receptor\u2010binding sites.<\/jats:p>","DOI":"10.1002\/2211-5463.12931","type":"journal-article","created":{"date-parts":[[2020,7,12]],"date-time":"2020-07-12T05:27:15Z","timestamp":1594531635000},"page":"1474-1481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High\u2010resolution crystal structures of the botulinum neurotoxin binding domains from subtypes A5 and A6"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4986-8594","authenticated-orcid":false,"given":"Jonathan R.","family":"Davies","sequence":"first","affiliation":[{"name":"Department of Biology and Biochemistry Claverton Down University of Bath Bath UK"},{"name":"Department of Biochemistry and Biophysics Stockholm University Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Britton","sequence":"additional","affiliation":[{"name":"Department of Biology and Biochemistry Claverton Down University of Bath Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6359-0229","authenticated-orcid":false,"given":"Sai Man","family":"Liu","sequence":"additional","affiliation":[{"name":"Ipsen Bioinnovation Limited Milton Park Abingdon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3009-4058","authenticated-orcid":false,"given":"K. 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