{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:16:52Z","timestamp":1786976212128,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771562"],"award-info":[{"award-number":["81771562"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971364"],"award-info":[{"award-number":["81971364"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>\n                    Loss of T\u2010type calcium channel (TCC) function has been reported to result in decreased cell viability and impaired muscle regeneration, but the underlying mechanisms remain largely unknown. We previously found that expression of TCC is reduced in aged pelvic floor muscle of multiple vaginal delivery mice, and this is related to endoplasmic reticulum stress (ERS) activation and autophagy flux blockade. In the present work, we further investigated the effects of TCC function loss on C2C12 myotubes and skeletal muscle, which is mediated by promotion of ERS and ultimately contributes to mitochondrial\u2010related apoptotic cell death. We found that application of a TCC inhibitor induced mitochondria\u2010related apoptosis in a dose\u2010dependent manner and also reduced mitochondrial transmembrane potential (MMP), induced mito\u2010ROS generation, and enhanced expression of mitochondrial apoptosis proteins. Functional inhibition of TCC induced ERS, resulting in disorder of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis in endoplasmic reticulum, and ultimately leading to cell apoptosis in C2C12 myotubes. Tibialis anterior muscles of T\u2010type \u03b11H channel knockout mice displayed a smaller skeletal muscle fiber size and elevated ERS\u2010mediated apoptosis signaling. Our data point to a novel mechanism whereby TCC blockade leads to ERS activation and terminal mitochondrial\u2010related apoptotic events in C2C12 myotubes and skeletal muscles.\n                  <\/jats:p>","DOI":"10.1002\/2211-5463.12965","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T08:39:41Z","timestamp":1598863181000},"page":"2122-2136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["T\u2010type calcium channel blockade induces apoptosis in C2C12 myotubes and skeletal muscle via endoplasmic reticulum stress activation"],"prefix":"10.1002","volume":"10","author":[{"given":"Mao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics Renmin Hospital of Wuhan University China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suting","family":"Li","sequence":"additional","affiliation":[{"name":"Department of 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