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Despite accumulating genomic, transcriptomic, proteomic, and lipidomic data on IPF, evidence from water\u2010soluble metabolomics is limited. To identify biomarkers for IPF from water\u2010soluble metabolomic data, we measured the levels of various metabolites in bronchoalveolar lavage fluid (BALF) and serum samples from a bleomycin\u2010induced murine pulmonary fibrotic model using gas chromatography\/mass spectrometry. Thirty\u2010two of 73 BALF metabolites and 29 of 74 serum metabolites were annotated. We observed that the levels of proline and methionine were higher in BALF but lower in serum than those in the control. Furthermore, analysis of public RNA\u2010Seq data from the lungs of patients with IPF revealed that proline\u2010 and methionine\u2010related genes were significantly upregulated compared to those in the lungs of healthy controls. These results suggest that proline and methionine may be potential biomarkers for IPF and may help to deepen our understanding of the pathophysiology of the disease. 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