{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T18:32:12Z","timestamp":1770489132629,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T00:00:00Z","timestamp":1601856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>Malignant mesothelioma (MM) is an aggressive malignant neoplasm which rapidly invades pleural tissues and has a poor prognosis. Here, we explore enhancement of the effect of irinotecan [camptothecin\u201011 (CPT\u201011)] by the p53\u2010dependent induction of carboxylesterase 2 (CES2), a CPT\u201011\u2010activating enzyme, in MM. The level of <jats:italic>CES2<\/jats:italic> mRNA was greatly increased on treatment with nutlin\u20103a. A combination of CPT\u201011 and nutlin\u20103a inhibited the growth of MM cells more effectively than either drug alone. Knocking down <jats:italic>CES2<\/jats:italic> in MM cells reduced the effect of the drug combination, and its forced expression in MESO4 cells enhanced the growth inhibitory activity of CPT\u201011 in the absence of nutlin\u20103a. Enhancement of the growth inhibitory activity of CPT\u201011 by nutlin\u20103a suggests a possible new combinatorial MM chemotherapy regimen.<\/jats:p>","DOI":"10.1002\/2211-5463.12985","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T01:07:22Z","timestamp":1600823242000},"page":"2375-2387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Combined use of irinotecan and p53 activator enhances growth inhibition of mesothelioma cells"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7814-0917","authenticated-orcid":false,"given":"Bo","family":"Han","sequence":"first","affiliation":[{"name":"Department of Molecular Pathogenesis Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5493-8112","authenticated-orcid":false,"given":"Hyeon\u2010Cheol","family":"Lee\u2010Okada","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momoko","family":"Ishimine","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Orita","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Minimally Invasive Surgery Juntendo University Faculty of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathogenesis Juntendo University Graduate School of Medicine Tokyo Japan"},{"name":"Department of Pathology and Oncology Juntendo University Faculty of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Takagaki","sequence":"additional","affiliation":[{"name":"Department of Pathology and Oncology Juntendo University Faculty of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Kajino","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathogenesis Juntendo University Graduate School of Medicine Tokyo Japan"},{"name":"Department of Pathology and Oncology Juntendo University Faculty of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiko","family":"Yokomizo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Okio","family":"Hino","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathogenesis Juntendo University Graduate School of Medicine Tokyo Japan"},{"name":"Department of Pathology and Oncology Juntendo University Faculty of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathogenesis Juntendo University Graduate School of Medicine Tokyo Japan"},{"name":"Department of Pathology and Oncology Juntendo University Faculty of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_10_3_1","volume-title":"WHO Classification of Tumours of the Lung, Pleura, Thymus and Heart","author":"Travis WD","year":"2015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-5002(01)00257-4"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000464410"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-53560-9_15"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2018.04.10"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.11.136"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2018.05.038"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00187"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2019.102815"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-1089"},{"key":"e_1_2_10_13_1","first-page":"1189","article-title":"Characterization of CPT\u201011 hydrolysis by human liver carboxylesterase isoforms hCE\u20101 and hCE\u20102","volume":"60","author":"Humerickhouse R","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_10_14_1","first-page":"423","article-title":"Pharmacokinetics, metabolism, and excretion of irinotecan (CPT\u201011) following I.V. infusion of [(14)C]CPT\u201011 in cancer patients","volume":"28","author":"Slatter JG","year":"2000","journal-title":"Drug Metab Dispos"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22366"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.5.11.3271"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12125"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5280736"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpbreports.2.6_130"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.158360"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601912454734"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25480"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.08.019"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3520"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2016.05.020"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-1184"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092472"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2192803"},{"key":"e_1_2_10_29_1","unstructured":"TP53 Database: International Agency for Research on Cancer.http:\/\/p53.iarc.fr\/celllines.aspx. Accessed 15 May 2018."},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401227"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2008.00936.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.30.5.541"},{"key":"e_1_2_10_33_1","first-page":"2605","article-title":"Human carboxylesterase 2 is commonly expressed in tumor tissue and is correlated with activation of irinotecan","volume":"8","author":"Xu G","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357900701560786"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01488.x"},{"key":"e_1_2_10_36_1","first-page":"4205","article-title":"Irinotecan and gemcitabine in patients with advanced non\u2010small cell lung cancer, previously treated with cisplatin\u2010based chemotherapy. A phase II study","volume":"23","author":"Pectasides D","year":"2003","journal-title":"Anticancer Res"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.033"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173022"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.04.009"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498254.2015.1082209"}],"container-title":["FEBS Open Bio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12985","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/2211-5463.12985","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/2211-5463.12985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T17:08:57Z","timestamp":1693847337000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1002\/2211-5463.12985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,5]]},"references-count":39,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1002\/2211-5463.12985"],"URL":"https:\/\/doi.org\/10.1002\/2211-5463.12985","archive":["Portico"],"relation":{},"ISSN":["2211-5463","2211-5463"],"issn-type":[{"value":"2211-5463","type":"print"},{"value":"2211-5463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,5]]},"assertion":[{"value":"2020-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}